1. Chapter 16 Worksheets Recombinant DNA lab Mini Labs Reinforcement & Study Guides Content Mastery Crossword

3. Bacteria use restriction enzymes to cut up viral DNA The ends join together with complimentary single strands on other split DNA molecules The cut below does not produce sticky ends, so the DNA fragment would not combine readily with other DNA fragments. Expected results: Students will examine how a specific restriction enzyme cuts a strand of DNA

4. Expected Results: Students will splice together the two different colors of wire, representing the insertion of a gene from a different species into a strand of DNA The wires represent DNA segments from two different species and the pieces of wire represent genes Stripping the wire is like using restriction enzymes to form sticky ends Splicing the wires together now allows the wires to function as a unit, and splicing a gene into a fragmented piece of DNA allows that gene to function within the DNA strand

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6. DNA fragment plasmid (vector) gene splicing Recombinant DNA plasmid containing bacterium cell division bacterial cell (host) clone How is cloning possible? What do genetic-engineering techniques provide?

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8. 100,000 have not linkage map sperm cystic fibrosis Electrophoresis It is an international effort to completely map & sequence the human genome. Because humans have only a few children compared to other species and the generation time is so long Prenatal diagnosis of human disorders, gene therapy, and DNA fingerprinting Because no two individuals with the exception of identical twins have the same DNA sequence, and all autosomal cells of an individual have the same DNA

9. clones human genome recombinant DNA plasmid cell culture vector transgenic organism gene therapy restriction enzyme linkage map genetic engineering gene splicing

11. Location A represents the longest fragment; location F represents the shortest fragment. Because smaller fragments of DNA move more quickly through the gel, the farther the standard marker band is from the well, the smaller is the fragment Father #2 might be the biological father because he matches a band with the child at F. Suspected father #1 does not match any bands with the child It would mean that the person is homozygous for the segment of DNA containing the repeated sequence. If the person is heterozygous, a separate band will be seen for each of the two alleles. However, if he or she is homozygous, there will be only one band visible Any combination of one band from the mother & one band from the father would be correct.