1. Biotechnology Practice Test

2. Question #1
How much material will a P-20 dispense if it reads 123?

3. Question #2
To join a piece of DNA from one source into another, which of these processes is used? a. Cloning b. Ligation c. Transformation d. Restriction digest e. DNA fingerprinting

4. Question #3 Which of these processes is used to cut up pieces of DNA?
Gene therapy
b. Ligation
c. Transformation
d. Restriction digest
e. DNA fingerprinting

5. Question #4
What is made by the work of restriction enzymes?

6. Question #5
Which of the following is NOT a way that bacteria obtain new combinations of genes?
Two bacteria exchange genetic info during conjugation
Bacteria produce sex cells that undergo fertilization
Viruses carry bacterial DNA from one bacteria to another
Bacteria take up free pieces of DNA from their environment

7. Question #6
DNA is loaded into a gel. What is the charge, if any, of the DNA?
Negative
Positive
Neutral
It depends on base pair length

8. Question #7
Which of these is in the correct order for making a protein from a recombinant?
Restriction digest, insertion, amplification, transformation
Restriction digest, ligation, transformation, amplification
Restriction digest, ligation, insertion, amplification
Transformation, restriction digest, ligation, amplification

9. Question #8
Which of the following would be considered a transgenic organism?
A plant grown in a lab from a single plant cell
b. A sheep developed from an egg cell that was fertilized in the lab
c. A sheep that produces a cow protein
d. A sheep developed from an egg cell that was fertilized in the lab

10. Question #9
The process that involves inserting correct forms of a gene into a vector, and putting them back into the body is known as _______.

11. Question #10
Why do both sources of DNA in a recombinant have to be cut with the same restriction enzyme?