1. Cloning a DNA segment from bacteriophage
Recombinant plasmid DNA purified from bacterial cells
Started restriction digests last week
Why digesting with EcoRI again?
Why digesting with EcoRI + BamHI?

2. Cloning a DNA segment from bacteriophage
Recombinant plasmid DNA purified from bacterial cells
LAB:
Retrieve samples
Add 5 µL of sample buffer (dye) - contains RNase
Load 15 µL of each digest onto agarose gel (you will share a gel)
Examine results - use restriction digest maps for EcoRI and BamHI

3. Cloning a DNA segment from bacteriophage
Restriction map of lambda DNA - EcoRI
Sizes of restriction fragments
21,226
4878
5643
7421
5804
3530
21,226
26,104
31,747
39,168
44,972
Position of EcoRI restriction sites
Restriction map of lambda DNA - BamHI
Sizes of restriction fragments
5505
16841
5626
6527
7233
6770
5505
22346
27972
34499
41732
Position of BamHI restriction sites