Relationship between Genotype and Phenotype Molecular Basis for Relationship between Genotype and Phenotype genotype DNA DNA sequence transcription RNA translation amino acid sequence protein function phenotype organism

Molecular Genetic Diagnostics: Sickle Cell Anemia Electron Micrograph HbA codes for normal b-globin and produces normal hemoglobin. HbS produces sickled red blood cells. Homozygotes for HbS are anemic. HbS produces b-globin that differs from normal protein by one amino acid.

Molecular Genetic Diagnostics: Sickle Cell Anemia

Molecular Genetic Diagnostics: Sickle Cell Anemia

Methods of introducting a transgene Solution of DNA to be studied is injected into host cell. Recombinant DNA can be delivered into host cell using a viral vector.

Methods of introducting a transgene Bombardment with DNA-coated tungsten particles introduces DNA into host cell. Under favorable conditions, DNA can be taken up by host cell by transformation.

Relationship between Genotype and Phenotype Molecular Basis for Relationship between Genotype and Phenotype genotype DNA DNA sequence transcription RNA translation amino acid sequence protein function phenotype organism

Transgenesis Introduction of foreign genetic material into a host organism. 2. Ability of the new genetic material to be transmitted to offspring of the host. 3. Potential of the new genetic material to be expressed in the host. 4. Transgenic animals or transgenic plants are the result of this process.

Ti (tumor-inducing) plasmid is 200-kb double-stranded circular DNA. Agrobacterium tumefaciens (soil bacterium) causes crown gall disease in infected plants. Ti (tumor-inducing) plasmid is 200-kb double-stranded circular DNA. T-DNA region is transferred and randomly inserted into host plant genome. T-DNA directs tumor production and synthesis of opines, compounds that the bacterium needs for growth.

Genetic Engineering in Plants using Ti Plasmid T-DNA can be replaced by DNA sequence of interest and introduced into host plant genome. Reporter genes (selectable markers) are spliced into the recombinant T-DNA. Successful integration is indicated by expression of reporter genes. Markers (reporter genes) such as kanamycin resistance gene are very useful in monitoring successful transformation.

Generation of a Transgenic Plant

Genetic Engineering in Plants Plants that have been transformed include horse-radish, morning glory, cucumber, carrot, yam, cotton, sunflower, lettuce, tomato, corn, soybean, alfalfa, tobacco, potato, and clover. Agriculturally important crops can be engineered to express: - viral resistance - pesticide resistance - herbicide resistance - insect resistance Nutritional value can be enhanced, for example, by introduction of genes that increase seed protein content. Plants can be engineered to produce pharmaceutically important proteins or vaccines. Luciferase gene is useful as a reporter gene for transformation.