Plan C 1.Pick a problem 2.Pick some plants to study 3.Design some experiments 4.See where they lead us
1.Biofuels What would make a good biofuel? How and where to grow it? Can we get plants to make diesel, H 2 (g) or electricity? 2.Climate/CO 2 change How will plants be affected? Can we use plants to help alleviate it? 3.Stress responses/stress avoidance Structural Biochemical (including C3 vs C4 vs CAM) Other (dormancy, carnivory, etc) 4.Plant products 5.Improving food production 6.Phytoremediation 7.Plant signaling (including neurobiology) 8.Something else?
Endomembrane system Organelles derived from the ER 1) ER 2) Golgi 3) Vacuoles 4) Plasma Membrane 5) Nuclear Envelope 6) Endosomes 7) Oleosomes
GOLGI COMPLEX Individual, flattened stacks of membranes made from ER Fn: “post office”: collect ER products, process & deliver them Altered in each stack Makes most cell wall carbohydrates! Protein’s address is built in
VACUOLES Derived from Golgi; Fns: 1)digestion a) Organelles b) food particles
VACUOLES Derived from Golgi; Fns: 1)digestion a) Organelles b) food particles 2) storage
VACUOLES Derived from Golgi; Fns: 1) digestion a) Organelles b) food particles 2) storage 3) turgor: push plasma membrane against cell wall
VACUOLES Vacuoles are subdivided: lytic vacuoles are distinct from storage vacuoles!
Endomembrane system Organelles derived from the ER 1) ER 2) Golgi 3) Vacuoles 4) Plasma Membrane Regulates transport in/out of cell
Endomembrane system Organelles derived from the ER 1) ER 2) Golgi 3) Vacuoles 4) Plasma Membrane Regulates transport in/out of cell Lipids form barrier Proteins transport objects & info
Endomembrane System 5) Nuclear envelope: regulates transport in/out of nucleus Continuous with ER
Endomembrane System 5) Nuclear envelope:regulates transport in/out of nucleus Continuous with ER Transport is only through nuclear pores
Endomembrane System 5) Nuclear envelope:regulates transport in/out of nucleus Continuous with ER Transport is only through nuclear pores Need correct signal & receptor for import
Endomembrane System 5) Nuclear envelope: regulates transport in/out of nucleus Continuous with ER Transport is only through nuclear pores Need correct signal & receptor for import new one for export
Endomembrane System Endosomes: vesicles derived from Golgi or Plasma membrane Fn: sorting materials & recycling receptors
Endomembrane System Oleosomes: oil storage bodies derived from SER Surrounded by lipid monolayer!
Endomembrane System Oleosomes: oil storage bodies derived from SER Surrounded by lipid monolayer! filled with lipids: no internal hydrophobic effect!
endosymbionts derived by division of preexisting organelles no vesicle transport Proteins & lipids are not glycosylated
endosymbionts derived by division of preexisting organelles little exchange of membranes with other organelles 1) Peroxisomes (microbodies)
Peroxisomes (microbodies) 1 membrane
Peroxisomes (microbodies) found in (nearly) all eukaryotes 1 membrane Fn: 1) destroy H 2 O 2, other O 2 -related poisons
Peroxisomes Fn: 1.destroy H 2 O 2, other O 2 -related poisons 2.change fat to CH 2 O (glyoxysomes)
Peroxisomes Fns: 1.destroy H 2 O 2, other O 2 -related poisons 2.change fat to CH 2 O (glyoxysomes) 3.Detoxify & recycle photorespiration products
Peroxisomes Fn: 1.destroy H 2 O 2, other O 2 -related poisons 2.change fat to CH 2 O (glyoxysomes) 3.Detoxify & recycle photorespiration products 4.Destroy EtOH (made in anaerobic roots)
Peroxisomes ER can make peroxisomes under special circumstances! e.g. peroxisome-less mutants can restore peroxisomes when the wild-type gene is restored
endosymbionts 1) Peroxisomes (microbodies) 2) Mitochondria
Mitochondria Bounded by 2 membranes
Mitochondria 2 membranes Smooth OM
Mitochondria 2 membranes Smooth OM IM folds into cristae
Mitochondria -> 4 compartments 1) OM 2) intermembrane space 3) IM 4) matrix
Mitochondria matrix contains DNA, RNA and ribosomes
Mitochondria matrix contains DNA, RNA and ribosomes Genomes vary from 100,000 to 2,500,000 bp, but only genes
Mitochondria matrix contains DNA, RNA and ribosomes Genomes vary from 100,000 to 2,500,000 bp, but only genes Reproduce by fission
Mitochondria matrix contains DNA, RNA and ribosomes Genomes vary from 100,000 to 2,500,000 bp, but only genes Reproduce by fission IM is 25% cardiolipin, a bacterial phospholipid
Mitochondria Genomes vary from 100,000 to 2,500,000 bp, but only genes Reproduce by fission IM is 25% cardiolipin, a bacterial phospholipid Genes most related to Rhodobacteria
Mitochondria Fn : cellular respiration -> oxidizing food & supplying energy to cell Also make many important biochemicals
Mitochondria Fn : cellular respiration -> oxidizing food & supplying energy to cell Also make important biochemicals & help recycle PR products
endosymbionts 1)Peroxisomes 2)Mitochondria 3) Plastids
Plastids Chloroplasts do photosynthesis Amyloplasts store starch Chromoplasts store pigments Leucoplasts are found in roots
Chloroplasts Bounded by 2 membranes 1) outer envelope 2) inner envelope
Chloroplasts Interior = stroma Contains thylakoids membranes where light rxns of photosynthesis occur mainly galactolipids
Chloroplasts Interior = stroma Contains thylakoids membranes where light rxns of photosynthesis occur mainly galactolipids Contain DNA, RNA, ribosomes
Chloroplasts Contain DNA, RNA, ribosomes 120, ,000 bp, ~ 100 genes
Chloroplasts Contain DNA, RNA, ribosomes 120, ,000 bp, ~ 100 genes Closest relatives = cyanobacteria
Chloroplasts Contain DNA, RNA, ribosomes 120, ,000 bp, ~ 100 genes Closest relatives = cyanobacteria Divide by fission
Chloroplasts Contain DNA, RNA, ribosomes 120, ,000 bp, ~ 100 genes Closest relatives = cyanobacteria Divide by fission Fns: Photosynthesis
Chloroplasts Fns: Photosynthesis & starch synth Photoassimilation of N & S
Chloroplasts Fns: Photosynthesis & starch synth Photoassimilation of N & S Fatty acid & some lipid synth
Chloroplasts Fns: Photosynthesis & starch synth Photoassimilation of N & S Fatty acid & some lipid synth Synth of ABA, GA, many other biochem
Chloroplasts & Mitochondria Contain eubacterial DNA, RNA, ribosomes Inner membranes have bacterial lipids Divide by fission Provide best support for endosymbiosis
Endosymbiosis theory (Margulis) Archaebacteria ate eubacteria & converted them to symbionts
Endosymbiosis theory (Margulis) Archaebacteria ate eubacteria & converted them to symbionts
Endosymbiosis theory (Margulis) Archaebacteria ate eubacteria & converted them to symbionts
cytoskeleton network of proteins which give cells their shape also responsible for shape of plant cells because guide cell wall formation left intact by detergents that extract rest of cell
Cytoskeleton Actin fibers (microfilaments) ~7 nm diameter Form 2 chains of polar actin subunits arranged in a double helix
Actin fibers polar subunits arranged in a double helix Add to + end Fall off - end Fn = movement
Actin fibers Very conserved in evolution Fn = motility Often with myosin
Actin fibers Very conserved in evolution Fn = motility Often with myosin: responsible for cytoplasmic streaming
Actin fibers Very conserved in evolution Fn = motility Often with myosin: responsible for cytoplasmic streaming, Pollen tube growth & movement through plasmodesmata
Actin fibers Often with myosin: responsible for cytoplasmic streaming, Pollen tube growth & movement through plasmodesmata
Intermediate filaments Protein fibers 8-12 nm dia (between MFs & MTs) form similar looking filaments Conserved central, rod-shaped -helical domain
Intermediate filaments 2 monomers form dimers with parallel subunits Dimers form tetramers aligned in opposite orientations & staggered
Intermediate filaments 2 monomers form dimers with parallel subunits Dimers form tetramers Tetramers form IF
Intermediate filaments 2 monomers form dimers with parallel subunits Dimers form tetramers Tetramers form IF Plants have several: Fn unclear
Microtubules Hollow, cylindrical; found in most eukaryotes outer diameter - 24 nm wall thickness - ~ 5 nm Made of 13 longitudinal rows of protofilaments
Microtubules Made of tubulin subunits polymerize to form protofilaments (PF) PF form sheets Sheets form microtubules
Microtubules Protofilaments are polar - end + end all in single MT have same polarity
Microtubules In constant flux polymerizing & depolymerizing Add to (+) Fall off (-)
Microtubules Control growth by controlling rates of assembly & disassembly because these are distinct processes can be controlled independently! Colchicine makes MTs disassemble Taxol prevents disassembly
Microtubules Control growth by controlling rates of assembly & disassembly Are constantly rearranging inside plant cells!
Microtubules Control growth by controlling rates of assembly & disassembly Are constantly rearranging inside plant cells! during mitosis & cytokinesis
Microtubules Control growth by controlling rates of assembly & disassembly Are constantly rearranging inside plant cells! during mitosis & cytokinesis Guide formation of cell plate & of walls in interphase
µT Assembly µTs always emerge from Microtubule-Organizing Centers (MTOC)
µT Assembly µTs always emerge from Microtubule-Organizing Centers (MTOC) patches of material at outer nuclear envelope
Microtubules MAPs (Microtubule Associated Proteins) may: stabilize tubules alter rates of assembly/disassembly crosslink adjacent tubules link cargo
2 classes of molecular motors 1) Kinesins move cargo to µT plus end 2) Dyneins move cargo to minus end “Walk” hand-over-hand towards chosen end
µT functions 1)Give cells shape by guiding cellulose synth
µT functions 1)Give cells shape by guiding cellulose synth 2)Anchor organelles
µT functions 1)Give cells shape by guiding cellulose synth 2)Anchor organelles 3)Intracellular motility