12.3 DNA, RNA, and Protein Objective: 6(C) Explain the purpose and process of transcription and translation using models of DNA and RNA.

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12.3 DNA, RNA, and Protein Objective: 6(C) Explain the purpose and process of transcription and translation using models of DNA and RNA.

2(G) Analyze, evaluate, make inferences, and predict trends from data. 4(B) Investigate and explain cellular processes, including homeostasis, energy conversions, transport of molecules, and synthesis of new molecules. 6(A) Identify components of DNA, and describe how information for specifying the traits of an organism is carried in the DNA. 6(B) Recognize that components that make up the genetic code are common to all organisms. DNA, RNA, and Protein Copyright © McGraw-Hill Education

Essential Questions How are messenger RNA, ribosomal RNA, and transfer RNA involved in the transcription and translation of genes? What is the role of RNA polymerase in the synthesis of messenger RNA? How is the code of DNA translated into messenger RNA and utilized to synthesize a protein? DNA, RNA, and Protein Copyright © McGraw-Hill Education

Review synthesis New RNA messenger RNA ribosomal RNA transfer RNA transcription RNA polymerase intron exon codon translation DNA, RNA, and Protein Copyright © McGraw-Hill Education Vocabulary

Central Dogma After the discovery of DNA’s structure, scientists turned to investigating how DNA served as a genetic code for the synthesis of proteins. Geneticists accept that the basic mechanism for reading and expressing genes is from DNA to RNA to protein. This is referred to as the central dogma of biology: DNA codes for RNA, which guides the synthesis of proteins. DNA, RNA, and Protein Copyright © McGraw-Hill Education

Central Dogma RNA RNA is a nucleic acid similar to DNA, but with a different sugar, and with uracil instead of thymine. Messenger RNA (mRNA): long strands of RNA that are formed complementary to one strand of DNA; direct synthesis of a specific protein Ribosomal RNA (rRNA): associates with proteins to form ribosomes in the cytoplasm Transfer RNA (tRNA): smaller segments of RNA that transport amino acids to the ribosome DNA, RNA, and Protein Copyright © McGraw-Hill Education

Central Dogma Transcription First step of the central dogma involves the synthesis of mRNA from DNA in a process called transcription. The enzyme RNA polymerase regulates RNA synthesis by binding to the specific section where an mRNA will be synthesized. RNA polymerase moves along the DNA strand in a 3’ to 5’ direction, synthesizing mRNA. DNA, RNA, and Protein Copyright © McGraw-Hill Education

Central Dogma RNA processing In comparing DNA code with its transcribed mRNA code, scientists found that the mRNA code was much shorter. DNA sequences not found in mRNA are called introns. DNA sequences that remain in the final mRNA are called exons. DNA, RNA, and Protein Copyright © McGraw-Hill Education

The Code Scientists hypothesized that the instructions from protein synthesis were encoded in DNA. Experiments during the 1960s demonstrated that the DNA code was a three-base code. The three-base code in DNA or mRNA is called a codon. DNA, RNA, and Protein Copyright © McGraw-Hill Education

The Code Translation After synthesis, mRNA moves from the nucleus into the cytoplasm, where it connects at the 5’ end to a ribosome. The mRNA code is read and translated into a protein through a process called translation. DNA, RNA, and Protein Copyright © McGraw-Hill Education

The Code Translation tRNA molecules act as the interpreters of the mRNA codon sequence. The tRNA is activated by an enzyme that attaches a specific amino acid to the 3’ end. The middle of the folded tRNA contains an anticodon, a complementary sequence to the mRNA codon. DNA, RNA, and Protein Copyright © McGraw-Hill Education

The Code The role of the ribosome Ribosomes provide a site for protein synthesis. When mRNA leaves the nucleus, the two ribosomal subunits come together to hold the mRNA in place for translation. The ribosome structure has grooves that hold serve as tRNA sites for amino acid attachment. DNA, RNA, and Protein Copyright © McGraw-Hill Education

One Gene – One Enzyme The Beadle and Tatum experiment showed that one gene codes for one enzyme. We now know that one gene codes for one polypeptide. DNA, RNA, and Protein Copyright © McGraw-Hill Education

DNA, RNA, and Protein Copyright © McGraw-Hill Education Review Essential Questions How are messenger RNA, ribosomal RNA, and transfer RNA involved in the transcription and translation of genes? What is the role of RNA polymerase in the synthesis of messenger RNA? How is the code of DNA translated into messenger RNA and utilized to synthesize a protein ? Vocabulary RNA messenger RNA ribosomal RNA transfer RNA transcription RNA polymerase intron exon codon translation