DNA Technology Chapter 11

Genetic Technology- Terms to Know Genetic engineering- Genetic engineering- Recombinant DNA- DNA made from 2 or more organisms Recombinant DNA- DNA made from 2 or more organisms Vector- What gets the gene into the cell Vector- What gets the gene into the cell Usually a virus, yeast, or plasmid Usually a virus, yeast, or plasmid Plasmid- circular bits of DNA Plasmid- circular bits of DNA Restriction enzymes- proteins which cut the DNA at specific points Restriction enzymes- proteins which cut the DNA at specific points

New Technology Human Genome Project- mapping entire human genome sequence. Finished in Human Genome Project- mapping entire human genome sequence. Finished in Cloning- process used to creating identical copy of organism Cloning- process used to creating identical copy of organism Polymerase Chain Reaction (PCR)- process that makes more DNA Polymerase Chain Reaction (PCR)- process that makes more DNA DNA Fingerprinting- use gel electrophoresis to separate DNA of different lengths DNA Fingerprinting- use gel electrophoresis to separate DNA of different lengths

Genetic Engineering Information Gel Electrophoresis Gel Electrophoresis Uses electric charges within gel Uses electric charges within gel DNA is negative, thus travels to positive end DNA is negative, thus travels to positive end Separates molecules by size Separates molecules by size

More New Technology Genetically Modified Foods- genetically selecting certain traits for crop improvement Genetically Modified Foods- genetically selecting certain traits for crop improvement Transgenic Animals- Animals that have other DNA in their cell. Used to make proteins, medicine, etc. Transgenic Animals- Animals that have other DNA in their cell. Used to make proteins, medicine, etc. Gene Therapy- insert genes into organism to help stop or prevent disease Gene Therapy- insert genes into organism to help stop or prevent disease

Remember… “There is no gene for Human Spirit!”