1. 5 10 15 20 25 The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25 5 10 15 20 25

2. The first adult mammal successfully cloned was a sheep named this. What is Dolly? 5

3. Dolly was cloned from what type of cell. What is a mammary cell? 10

4. These are the two most common DNA cloning vectors. 15 What are plasmids and bacteriophages?

5. Dolly was created using this type of cloning technique. What is nuclear transfer? 20

6. Cloned goats are used to produce this substance for human use. 25 What is clotting factor?

7. The Human Genome Project was completed in this year. 5 What is 2003?

8. The Human Genome Project discovered that humans have this many genes. What is 30,000 to 40,000? 10

9. This is an organism’s complete set of proteins. What is a proteome? 15

10. This percentage of human DNA differs from person to person. What is 0.1%? 20

11. The Human Genome Project revealed 8 million loci where individuals differ by only one nucleotide. These are known as this. What are SNPS (single nucleotide polymorphisms? 25

12. These are enzymes that cut DNA molecules at specific places. 5 W hat are restriction Enzymes?

13. These are circular pieces of bacterial DNA that replicate and can be used as recombinant DNA vectors in genetic engineering. What are plasmids? 10

14. Fragments of DNA can join with each other if they have complementary sequences of bases on their ends known as this. What are sticky ends? 15

15. This human protein needed by diabetics is produced through recombinant DNA technology. What is insulin? 20

16. Recombinant DNA means that the DNA from two different ones of these have been combined. What is species? 25

17. What is size? 5 Gel Electrophoresis sorts DNA fragments according to this.

18. Gel Electrophoresis makes use of the fact that DNA molecules have this overall charge. What is negative? 10

19. DNA will move towards what end of the chamber? What is the positive end? 15

20. The gel in gel electrophoresis is made of this substance. What is agarose? 20

21. This is the name of the solution that is poured into the chamber. What is TBE buffer? 25

22. This is the first step in DNA fingerprinting? What is isolate a DNA sample? 5

23. If a sample of DNA found at a crime scene is very small, this technique could be used to amplify (or make multiple copies) of it. What is PCR (Polymerase Chain Reaction)? 10

24. DNA fingerprinting makes use of these segments of repeating DNA nucleotides? What is VNTRs (Variable number tandem repeats)? 15

25. In DNA fingerprinting, cut DNA fragments are sorted using this technology. What is Gel Electrophoresis? 20

26. The FBI uses this many VNTRs to make a positive DNA match. What is 13? 25