Genomics and Proteomics. DNA sequencing: dideoxy chain termination.

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Presentation transcript:

Genomics and Proteomics

DNA sequencing: dideoxy chain termination

Sequencing entire genomes

DNA Analysis

A Molecular Description of a Gene

DNA Analysis

BLAST: A tool for annotation and characterization

Genomics based approach in Arabidopsis *Arabidopsis genome sequenced in 2000 Rat r-protein sequences BLAST algorithm search used to find Arabidopsis ribosomal protein genes Barakat, A. et al. (2001) Plant Physiology vol. 127, pp Total genes 80 Total putative r-proteins

rpl7-2 rps17-1, rps17-2 rps16-1 rps25-1 rps4-1, rpl8-1 rpl14-1 rps30-1, rps15a-2 rpl28-1, rpl31-1 rps25-2* rpl4-3* rpl39-1 rpp2-1, rpp2-2 rpl10a-2 rps12-3 rps3-1, rpl27-1 rpl23-2 rpl24-1 rps14-1, rpl40-1 rpl18a-2, rpl37a-1* rps5-1, rpl36-1 rpl23a-1, rpl35-2 rps15a-3 rps26-1, rps26-2 rpp0-1, rpl41-3 rps2-3, rpl11-1, rpl38-1 rpl7-3 rps27-1 rps27a-2 rpl7a-1, rpl18-1 rpl12-1 rpl35-4, rpl36-3 rps23-2, rpl4-4 rps28-2 rps17-4 rps4-2 rps15-2, rps15-3, rps15-4 rps6-2 rps9-2 rps7-3, rps16-3, rps19-2 rps8-1, rpl10a-3 rps11-3, rpl13-4 rps21-3, rpl22-3 rps24-2, rpl18-3 rps3-3 rps4-3* rpp2-5, rpl5-2 rps9-3, rpl5-3* rps10-2 rpl3-3* rps15-5 rpl32-2 rpl11-4 rps27-4, rpp1-3 rpl13a-4 rps10-3 rps4-4, rps8-2, rpp3-2 rps30-3, rpl31-3 rps15a-6, rpl12-3 rps19-3, rps20-3 rps15-6* rps28-3 rpl26-2 rps13-2 rpp1-2 rpl19-3 rpl9-4 rps18-3 rps9-1* rpl13a-3 rpl27-3 rpl36a-2 rpl15-1 rpl15-2 rpl32-1 rpl11-3 rpl14-2, rpl31-2 rps10-1, rpp3-1 rps30-2, rpl28-3 rps11-2, rps15a-5 rps6-1, rpl39-3 rps3a-2, rps25-4* rps29-3, rps29-4* rpl8-3 rps25-5 rpl13a-1,rpl29-1, rpl29-2 rpl13a-1, rpl29-1, rpl29-2 rps21-2, rpl36-2 rpl23a-2, rps2-4 rpl11-2, rpl21-6* rps26-3, rpl35a-4, rpl41-7 rps27a-3 rpl38-2, rpl37a-3, rps13-1 rps27-2, rps27-3* rpl17-2 rps7-2, rps19-1, rpl39-2 rps3a-1, rps24-1 rpsa-2, rps16-2, rpl23-3 rpl41-4 rpl18-2, rpl22-2 rpl37a-2, rpl41-5 rps14-2, rps17-3 rps5-2, rpp0-2 rpp0-3 rps28-1 rpl7-4, rpl18a-3 rpl19-2, rpl37-3 rpl30-3 rpl27-2 rpl36a-1 rpl5-1, rpl13a-2 rps21-1* rpp2-3, rpl34-3 rps25-3* rps20-1 rps29-1, rps29-2 rps15a-4, rpp2-4 rps20-2 rpl13-1* rpl13-2, rpl13-3 rps11-1 rpl8-2, rpl26-1 rps14-3, rpl40-2 rps3-3, rpl12-2 rpl24-2 rpl35-3 rpl28-2*, rpl41-6 rpl10a-1 rpp1-1,rpl23-1, rps15-1 rpp1-1, rpl23-1, rps15-1 rpl19-1, rpl22-1 rps15a-1, rpl35a-1 rpl21-1, rpl21-2*,rpl21-3 rpl21-1, rpl21-2*, rpl21-3 rpl10-1 rpl27a-1, rpl14-1 rpl35-1, rps23-1 rpl37-1, rps12-1 rpl6-1 rpl27a-2, rps18-1, rps27a-1 rpl10-2, rpl34-1 rpl18a-1 rpl21-4* rpl17-1 rpl19-1,rpl19-2, rpl19-3 rpl19-1, rpl19-2, rpl19-3 rps18-2 rpl4-2* rpl35a-2, rpl30-1 rpl3-1 rps7-1 rpl37-2 rpl41-1, rpl21-5 rps2-1 rpl3-2, rps2-2 rpl10-3, rpl17-2 rpl27a-3, rpl34-2 rpsa-1 rpl6-2, rpl6-3 rpl35a-3 rpl30-2 rpl41-2* rps12-2* rpl cM kb/ r-protein 436 kb/ r-protein 331 kb/ r-protein 605 kb/ r-protein 519 kb/ r-protein

Microarrays (“Gene chips”)

Transcriptional Response of Human Fibroblasts to Serum