1. Saccharomyces Genome Database (SGD)
Rama Balakrishnan
AmiGO Tutorial
Saccharomyces Genome Database (SGD)
Stanford University

2. What is AmiGO? Web application that reads from the GO Database
Allows to
browse the ontologies
annotations from various species
compare sequences (Gost)

3. AmiGO http://www.godatabase.org
Graphical View

4. Some basics Is_a relationship Part_of relationship
Node has children, can be clicked to view children
Node has been opened, can be clicked to close
Leaf node or no children

5. Browsing the Ontologies

6. Click on the term name to view term details and annotations

7. Term details
links to representations of this term in other databases
Annotations from various species

8. Term Tree View

9. Annotations associated with a term
Annotation data are from the gene_associations file submitted by the annotating groups

10. Graphical View for the term: DNA replication

11. Searching by gene product name

12. Advanced search

13. GOST-Gene Ontology blaST
Blast a protein sequence against all gene products that have a GO annotation.

14. GOst