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Correction of translational start site by identification of N-terminal peptide. Correction of translational start site by identification of N-terminal.

Published byGlenna Sudjarwadi Modified over 5 years ago

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Presentation on theme: "Correction of translational start site by identification of N-terminal peptide. Correction of translational start site by identification of N-terminal."— Presentation transcript:

1 Correction of translational start site by identification of N-terminal peptide.
Correction of translational start site by identification of N-terminal peptide.A, A Peptide GDASLTTELGR with a nontryptic N terminus was mapped downstream of the annotated translational start site of the gene Rv1106c. Gene model by Gene prediction program FgeneSB and orthologous protein from M. tuberculosis Sumu004 support the re-annotation of the TSS by shortening protein length by 4 amino acids. B, Protein sequence of Rv1106c is shown. Text in gray indicates amino acids removed in the revised gene model for Rv1106c. C, A representative MS/MS spectrum for the peptide GDASLTTELGR is shown. Dhanashree S. Kelkar et al. Mol Cell Proteomics 2011;10:M © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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Percentage of proteins identified in envelope membrane extracts according to the purification method and the number of transmembrane domains. Percentage.
Novel phosphorylation sites on H+-ATPase proteins

Novel phosphorylation sites on H+-ATPase proteins
A, high resolution MS/MS spectrum (lower panel) of 1435

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Protein information in the Human Protein Atlas.

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