H.Ghaderian1 1-Deparyment of Microbiology , Faculty of Biological Sciences , Islamic Azad Univercity , Falanarjan Branch , 804515/155 , Esfahan , Iran

:Classification Family : Herpes viridea Sub family : herpes virinea Genus : simplex Specieas:HSV-1CL 101 ,HSV-2 strain KN 53690 common name : herpes symplex virus

tegument

Alpha herpes viruses latently infected

Herpes virus in the host cell

HSV of the newborn is acquired 3 periods: in utera Peripartium (85%) postpartum

Incidence of neonatal herpes in 3 form : 1) lesion of the skin , eyes , mouth

2) Encephalitis with or without lesion

3) Dissiminated desease in liver lunges gland adrenl gut

Neonatal herpes case of neonatal herpes HSV -1and HSV-2 Investigation showed Neonatal disseminated HSV infection is most frequently caused by HSV-2, although HSV-1 can also be the cause Serologic investigations showed no IgM and IgG antibodies for HSV-1 and HSV-2 in new born

Immune ResponsesNeonatal Infected neonates produce HSV-specific IgM antibodies within 3 weeks of acquisition of the viral infection, which increase rapidly during the first 2 to 3 months and may remain for as long as 1 year after neonatal infection develops.

Lymphocytes from infected infants alpha-interferon Neonates INF –αcompare with adults with primary HSV infection is decreased.

Study showed : The first time infection of the pregnant women may lead to severe illness in pregnancy and may be virus transmission to newborn.

DIAGNISTIC 1)PCR The high risk for death requires prompt diagnostic therfore suggestive that of HSV was identified by PCR Viral DNA was extracted from all specimens (tracheal aspirate, liver, lungs, and gut) By using the QIAamp DNA Mini Kit

primers for the HSV-1: thymidinekinase gene ((Fw 5′-AGCGTCTTGTCATTGGCGAA-3′ (Rev 5′-TTTTCTGCTCCAGGCGGACT-3′) Primer for the HSV-2: DNA polymerase gene ((Fw 5′-CGTCCTGGAGTTTGACAGCG-3′ (Rev 5′-CAGCAGCGAGTCCTGCACACAA-3′) The DNA was then used for HSV DNAPCR

Nucleotide sequence analysis showed identical sequences in different specimens. GenBank BLAST tool highest similarity was HSV-1 strain CL 101 and HSV-2 strain KN 53690

2)Cell culture • Multi nucleated epithelial cells

treatment All infants with diagnosed HSV infection must be treated with an intravenous therapy with acyclovir (60 mg/kg/day) Antibody Therapy Antibodies against the surface glycoproteins gB and gD prophylactic and therapeutic agents in HSV infection

Monophosphte acyclovoir mechanism acyclovir inactive acyclovir Viral thimydin kinas Monophosphte acyclovoir cellular thimydin kinase active )) acyclovir three phosphate is analog to guanin blocked DNApolymerasefunction

PREVENTION Cesarean Delivery cesarean delivery in a woman with active genital lesions can reduce risk of acquiring HSV to infant Antiviral Prophylaxis During Pregnancy the use of oral acyclovir near the end of pregnancy to suppress genital HSV Because of acyclovir’s safety

Hsv vaccine HSV-2 : glycoprotein D subunit vaccine adjuvanted :monophosphorylipid A(MPL) efficacy was limited to women who were HSV-1 and -2 seronegative before receiving vaccination.

Thank you for your Attention