Biotechnology Practice Test

1. An organism’s chromosomes are part of its ___. A.Plasmid B.Recombinant DNA C.Genome D.Enzymes

2. How many microliters will a P-20 pipetter dispense if it reads 005?

3. If you wanted to confirm that you successfully cut a plasmid into several pieces, which of technique would you use? A.Ligation B.Transformation C.Cloning D.Gel electrophoresis E.Restriction digest

4. What is formed as a result of using restriction enzymes? A.Recombinant DNA B.Sticky ends C.Many copies of a gene D.Recombinant plasmid

5. This process puts recombinant plasmids into bacterial cells. A.Amplification B.Ligation C.Restriction digest D.Transformation

6. How does a restriction enzyme know where to cut on a piece of DNA? A.It finds the right recognition sequence. B.It matches complementary bases. C.It uses DNA ligase. D.It uses gel electrophoresis.

7. The process of making two organisms with the exact DNA is known as ___. A.Gene therapy B.Cloning C.Recombinant technology D.Transformation E.Ligation

8. Which of the following would be considered a transgenic organism? A.A cat that is given a gene that makes dog ears B.Human treated with insulin made in bacteria C.A sheep that developed from a lab- fertilized egg D.A plant grown in a lab from a single plant cell

9. Which of these is FALSE about viruses? A.They inject DNA into host cells. B.They can replicate recombinant plasmids. C.They are used to carry genes into cells. D.They trick host cells into making viral proteins.

10. The process that involves inserting a correct gene into a virus then giving that virus to a person is known as ___. A.Gene therapy B.Cloning C.Transformation D.Recombinant DNA

11. Why do both sources of DNA in a recombinant have to be cut with the same restriction enzyme?