SuperArray Bioscience Corporation June 2003 Updates New Product Lines: MultiGene-12™ RT-PCR Profiling Kits SureSilencing™ siRNA Kits

PCR & RT-PCR Related Products: MultiGene-12™ RT-PCR Expression Profiling Kits New Array Verification and Gene Expression Profiling Methods from SuperArray

Advantages of MultiGene-12™ RT-PCR Profiling Kits Pathway-specific sets of genes Profiles eleven closely related genes in a single analysis Processes up to eight experiments simultaneously Ideal for multi-gene profiling and array verification Contains carefully designed and tested primers Provides a simple way to multiplex RT-PCR analyses

MultiGene-12™ Kit Contents Kit Contents: PCR-1000A: ReactionReady™ PCR master mix 1000 –Lyophilized Powder for 96 Reactions (8 tubes, 12 reactions per tube) –Taq DNA Polymerase, Buffer, Nucleotides (dNTPs) –Gel loading dye for end point analysis by agarose gel electrophoresis MultiGene-12™ Primer Strips: –Eight identical 12-well strips. Each tube represents one gene. –Eleven closely-related genes from one biological pathway –Twelfth tube contains GAPD for normalization

Additional Materials Required Accessory Products (Sold Separately): C-01: ReactionReady™ First Strand cDNA Synthesis Kit* Enough reagents for 8 reverse transcription reactions Buffers RI, RE, P, BC, H 2 O * At this time, not available in all international territories. Or Individual Components from Promega: ReagentProduct Random PrimersPromega Catalog # C118 RNase InhibitorPromega Catalog # N2511 Reverse TranscriptasePromega Catalog # M1701 dNTPsPromega Catalog # U1330

Overview of MultiGene-12™ RT-PCR Profiling Kit Procedure PCR C o C, 60 min ReactionReady™ First Strand cDNA Synthesis Kit* * At this time, not available in all international territories. Experimental RNA aliquot ReactionReady™ PCR master mix 1000 PCR-1000A Forward and Reverse Primer Sets for Individual Genes Experiments PH-nnn or PM-nnn Gel Analysis

Initial MultiGene-12™ Product Offering MultiGene-12™ Human Caspase RT-PCR Profiling Kit PH-001A CASP 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 13 MultiGene-12™ Human BCL2 RT-PCR Profiling Kit PH-002A BAD, BAK1, BAX, BCL2, BCL10, BCL2A1, BCL2L2, BCLxL, BCLxS, BNIP3, BIK MultiGene-12™ Human Integrin-I RT-PCR Profiling Kit PH-003A ITGA 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 2B MultiGene-12™ Human Integrin-II RT-PCR Profiling Kit PH-004A ITGA 11, ITGAL, ITGAM, ITGAV, ITGAX, ITGB1, 2, 3, 4, 5, 6

Initial MultiGene-12™ Product Offering MultiGene-12™ Human MMP-I RT-PCR Profiling Kit PH-005A MMP 1, 2, 3, 7, 8, 9, 10, 11, 12, 13, 14 MultiGene-12™ Human MMP-II RT-PCR Profiling Kit PH-006A MMP 15, 16, 17, 20, 24, 26, MMPL1, TIMP1, 2, 3, 4 MultiGene-12™ Human Cytokine-I RT-PCR Profiling Kit PH-007A IL1B, 2, 4, 5, 6, 8, 11, 12A, 13, 15 MultiGene-12™ Human Cytokine-II RT-PCR Profiling Kit PH-008A IL16, 17, 18, 25, IFNG, IL1A, CSF2, MIF, TGFB1, 3, LTA

MultiGene-12™ Products Coming Soon Equivalent Products for Mouse –PM-001 through PM-008 –MultiGene-12™ Mouse RT-PCR Profiling Kit Eight new products every month –Human and Mouse products released on alternate months

Differential Gene Expression Observed with a MultiGene-12™ RT-PCR Profiling Kit MultiGene-12™ Human Cytokine II RT-PCR Profiling Kit IL16 IL17 IL18 IL25 IFN  IL1A CSF2 MIF TGF  1 TGF  LTA GAPD Stimulated With PMA and A23187 Unstimulated RNA from Jurkat cells:

RNA Interference Products: SureSilencing™ siRNA Kits Knock down the expression of key genes in specific biological pathways

The siRNA Concept Hutvagner and Zamore (2002) Curr. Opin. Genet. Dev. 12:

How we generate siRNA Populations PCR T7 RNA Polymerase RNase III siRNA T7 Promoter Gene-specific cDNA fragments Same ones used for our GEArrays

Overview of SureSilencing™ siRNA Kit Procedure Negative Control siRNA SureSilencing™ siRNA LipofectAMINE2000™ (Invitrogen) transient transfection CELLS Harvest cells for RNA. Perform RT-PCR Verification. Verify decrease in message level of specific gene relative to negative control sample. Perform other biochemical, molecular or cell biological assay. 24 to 48 h incubation

Advantages of SureSilencing™ siRNA Kits Knock down key genes in specific pathways Pre-tested & validated. Over 70% suppression. Ready to use for transfection No enzymatic steps required Includes negative control siRNA Includes primers to verify down-regulated by RT-PCR

SureSilencing™ siRNA Kit Contents Kit Contents: –Gene-Specific siRNA population To known down expression of gene by at least 70% Enough for 90 transfections (24-well format) –Negative Control siRNA population To control for non-specific effects To normalize other assay results Enough for 18 transfections (24-well format) –Gene-Specific Primer Pair To verify decrease in gene expression Enough for 100 polymerase chain reactions

Additional Materials Required Accessory Products (Sold Separately): C-01: ReactionReady™ First Strand cDNA Synthesis Kit* Enough reagents for 8 reverse transcription reactions Buffers RI, RE, P, BC, H 2 O P-1000A: ReactionReady™ PCR master mix 1000 Enough lyophilized master mix for 96 reactions Eight tubes, twelve reactions each * At this time, not available in all international territories. Or Individual Components from Promega: ReagentProduct Random PrimersPromega Catalog # C118 RNase InhibitorPromega Catalog # N2511 Reverse TranscriptasePromega Catalog # M1701 dNTPsPromega Catalog # U1330 Taq DNA PolymerasePromega Catalog # M2661

Initial SureSilencing™ Product Offering RH-0001APCAdenomatosis polyposis coli RH-0002YY1YY1 transcription factor RH-0003E2F1E2F transcription factor 1 RH-0004BRCA1Breast cancer 1, early onset RH-0005BADBCL2-antagonist of cell death RH-0006MYCc-myc proto-oncogene RH-0007STAT1Signal transducer and activator of transcription 1 RH-0008FADDFas-associated via death domain RH-0009BCL2B-cell CLL/lymphoma 2 RH-0010LCKLymphocyte-Specific Protein Tyrosine Kinase Product base could potential be extended to most of our gene list. Priority assigned to key upstream regulators of biological pathways.

SureSilencing™ Products Coming Soon Product base could potential be extended to most of our gene list. Priority assigned to key upstream regulators of biological pathways. NF  B1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 CDKN2ACyclin-dependent kinase inhibitor 2A EGFREpidermal growth factor receptor MAPK1Mitogen-activated protein kinase 1

Relationship Between SuperArray Product Lines GEArray™ cDNA Arrays Kits MultiGene-12™ RT-PCR Profiling Kits SureSilencing™ siRNA Kits