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Cytokine expression induced by ligated-intestinal-loop assay using RT-PCR. Cytokine expression induced by ligated-intestinal-loop assay using RT-PCR. PCR.

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Presentation on theme: "Cytokine expression induced by ligated-intestinal-loop assay using RT-PCR. Cytokine expression induced by ligated-intestinal-loop assay using RT-PCR. PCR."— Presentation transcript:

1 Cytokine expression induced by ligated-intestinal-loop assay using RT-PCR.
Cytokine expression induced by ligated-intestinal-loop assay using RT-PCR. PCR products were applied to 2% agarose gels. Eleven kinds of cytokines (IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, and IL-12p35 [lanes 1B to 12]; IFN-γ [IFN]; TNF-α [TNF]; TGF-β [TGF]; and MIP-2 [MIP]) expressed in PP cells (a) and LP cells (b) were detected by RT-PCR using specific primer pairs. As an internal control, a DNA fragment of β-actin (actin) was also amplified. The data represent at least three separate experiments. Akinobu Kajikawa et al. Clin. Vaccine Immunol. 2010; doi: /CVI

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