Safe handling of enzymes Title slide Edit: Add presentation title and speaker(s). Editing slides in the Novozymes template Edit: All graphics are created exclusively for Microsoft PowerPoint. All objects, charts, tables, etc. are editable in PowerPoint and generally in Microsoft Office. Use the basic PowerPoint editing practice: Copy, Paste, Delete, Position, Change colours, lines, etc.. About the Guide: All slides contain guides to easy editing. guide Safe handling of enzymes 17/04/2017

AGENDA WHAT ARE ENZYMES? POTENTIAL RISKS OF ENZYME EXPOSURE 4/17/2017 NOVOZYMES PRESENTATION AGENDA WHAT ARE ENZYMES? POTENTIAL RISKS OF ENZYME EXPOSURE REDUCTION OF ENZYME EXPOSURE

What are Enzymes? Special kind of proteins found in all living matter. 17/04/2017 NOVOZYMES PRESENTATION Organogram Use: To illustrate organisation, management, competencies etc. Edit: This slide uses Connectors (Auto Shapes). Lines are connected with boxes. Move the position of the boxes and the lines will still be connected to the original position. Copy/Paste objects and lines for extra elements. Help: For more info search Help (Topmenu) keyword Connectors. guide What are Enzymes? Special kind of proteins found in all living matter. Vital for life, but not living substances themselves Catalysts - accelerate chemical reactions Specific and efficient in very small amounts In laundry detergents they break up stains so they can be washed away more easily

AGENDA WHAT ARE ENZYMES? POTENTIAL RISKS OF ENZYME EXPOSURE 4/17/2017 NOVOZYMES PRESENTATION AGENDA WHAT ARE ENZYMES? POTENTIAL RISKS OF ENZYME EXPOSURE REDUCTION OF ENZYME EXPOSURE

Potential Adverse Effects of Enzymes 17/04/2017 NOVOZYMES PRESENTATION Large text animation Use: Drawing attention to essential text after drawing attention to animated bullet points. Animation: Auto Spray movement/Essential text visible after bullet points. Duration 5 sec. guide Potential Adverse Effects of Enzymes Primary Irritation (eyes, skin, mucous membranes) Proteases Allergy Only through inhalation (not through skin contact) Like allergy to other soluble proteins (pollen, mold, animal dander) INDUSTRIAL ENZYMES ARE NOT TOXIC

Prevention of Skin and Eye Irritation 17/04/2017 NOVOZYMES PRESENTATION Process Illustration 3 Use: For illustration of processes. guide Prevention of Skin and Eye Irritation Keep high personal hygiene standard Avoid direct contact with enzymes Use personal protective equipment Moisture increases irritation Goggles and face shields Gloves NOVOZYMES PRESENTATION NOVOZYMES A/S

Main Causes of Allergy Development 17/04/2017 NOVOZYMES PRESENTATION Process Illustration 3 Use: For illustration of processes. guide Main Causes of Allergy Development Exposure pattern Individual predisposition Physical form of the allergen Genetically (“atopics”) Smoking habits Particle size Level Time Frequency NOVOZYMES PRESENTATION NOVOZYMES A/S

17/04/2017 NOVOZYMES PRESENTATION Basic Use: This is the basic slide with no extra Novozymes graphics added. Edit: In the top left corner you find Slide no., Date and Header. Change settings in the menu: > View > Header and Footer guide Allergens Allergens (e.g. pollen, house dust mites, molds, hair from dog/cat, etc. ) are proteins. Proteins in the form of dust may cause allergy when inhaled. Enzymes are proteins. Enzymes may therefore also cause allergy when inhaled.

NOVOZYMES PRESENTATION 17/04/2017 NOVOZYMES PRESENTATION Process Arrow Use: To illustrate projects and process development. Edit: Insert text with symbols. Edit position colours with PowerPoint. guide Allergy Development ALLERGENS DUST CONTROL SENSITIZATION MEDICAL SURVAILLANCE SYMPTOMS NOVOZYMES PRESENTATION NOVOZYMES A/S

Dust Control Personal protection Equipment Personal Hygiene Training 17/04/2017 NOVOZYMES PRESENTATION Dust Control Personal protection Equipment Face masks, Respirators Goggles, Gloves Full Body Protection Personal Hygiene Shower Clothing Training Procedures Allergy Information

Sensitization vs. Allergy 17/04/2017 NOVOZYMES PRESENTATION Process Illustration 2 Use: For illustration of processes. guide Sensitization vs. Allergy Sensitization Your body makes specific antibodies against the enzyme No symptoms Allergy After repeated enzyme exposure Antibodies cause symptoms Symptoms are immediate or delayed Symptoms are reversible NOVOZYMES PRESENTATION NOVOZYMES A/S

Allergy Sensitization: 17/04/2017 NOVOZYMES PRESENTATION Organogram Use: To illustrate organisation, management, competencies etc. Edit: This slide uses Connectors (Auto Shapes). Lines are connected with boxes. Move the position of the boxes and the lines will still be connected to the original position. Copy/Paste objects and lines for extra elements. Help: For more info search Help (Topmenu) keyword Connectors. guide Allergy Sensitization: A state where a person has developed specific antibodies to an allergen, e.g. pollen, flour, etc… Can be detected by “Skin Prick Test” or RAST Without symptoms is not a disease Is induced by inhalation of allergens, e.g. dust Sensitization is lifelong! DEFINITIONS AND CLARIFI-CATIONS

Allergy Allergy: Some sensitized people develop allergy symptoms 17/04/2017 NOVOZYMES PRESENTATION Organogram Use: To illustrate organisation, management, competencies etc. Edit: This slide uses Connectors (Auto Shapes). Lines are connected with boxes. Move the position of the boxes and the lines will still be connected to the original position. Copy/Paste objects and lines for extra elements. Help: For more info search Help (Topmenu) keyword Connectors. guide Allergy Allergy: Some sensitized people develop allergy symptoms Asthma or Hay-Fever Symptoms disappear when out of exposure Symptoms reoccur when re-exposed Enzyme allergy is a occupational disease DEFINITIONS AND CLARIFI-CATIONS

Allergy symptoms Itching & irritation of eyes Sneezing 17/04/2017 NOVOZYMES PRESENTATION Organogram Use: To illustrate organisation, management, competencies etc. Edit: This slide uses Connectors (Auto Shapes). Lines are connected with boxes. Move the position of the boxes and the lines will still be connected to the original position. Copy/Paste objects and lines for extra elements. Help: For more info search Help (Topmenu) keyword Connectors. guide Allergy symptoms Itching & irritation of eyes Sneezing Nasal or sinus congestion Coughing Tightness of chest Hoarseness Shortness of breath Asthma

AGENDA WHAT ARE ENZYMES? POTENTIAL RISKS OF ENZYME EXPOSURE 4/17/2017 NOVOZYMES PRESENTATION AGENDA WHAT ARE ENZYMES? POTENTIAL RISKS OF ENZYME EXPOSURE REDUCTION OF ENZYME EXPOSURE

Enzyme Safety Program Goal Elements of the program 17/04/2017 NOVOZYMES PRESENTATION CONCEPT: MOVING SPRAY ANIMATION EXPERIENCE: RUNS AUTOMATIC FROM BEGINING OF SLIDE ANIMATION TIME: 5 SEC. POSSIBLE TO CHANGE COLOURS OF THE SPARY Enzyme Safety Program Goal To prevent sensitization and allergic reactions by controlling inhalation of dust and aerosols Elements of the program Enzyme product formulation Engineering control Good work practices

Enzyme Product Formulation 17/04/2017 NOVOZYMES PRESENTATION Process Illustration 3 Use: For illustration of processes. guide Enzyme Product Formulation Liquids Powders Granulates (encapsulated/ coated enzymes) Very high potential for dust generation Use of powders should be avoided Low dust generation Potential dust generation from crushed granulates Potential aerosol generation Potential dust generation from dried spills NOVOZYMES PRESENTATION NOVOZYMES A/S

17/04/2017 NOVOZYMES PRESENTATION Pie Use: This chart uses the standard Novozymes settings and is a basic PowerPoint chart. Pie colours are set with a gradient in effects. guide Engineering Control Systems, processes, or equipment that reduce dust or aerosols at the source The primary way that dust and aerosols are controlled Tell your supervisor/safety officer of any ideas for improving the control of dust and aerosols

Types of Engineering Controls Contained handling systems 17/04/2017 NOVOZYMES PRESENTATION Pie Use: This chart uses the standard Novozymes settings and is a basic PowerPoint chart. Pie colours are set with a gradient in effects. guide Engineering Control Types of Engineering Controls Contained handling systems Avoid conveyors that may break up enzymes Enclosures Enclose operations such as filling stations that have the potential to generate dust or aerosols Local exhaust ventilation Use for operators where dust and aerosols are unavoidable

4/17/2017 NOVOZYMES PRESENTATION Good Work Practices 1 Avoid direct contact with enzymes and enzyme contamination on clothes Avoid generation of dust or aerosols Remove spills immediately No sweeping or high pressure washing Vacuum only with HEPA filters Moisten to remove completely

4/17/2017 NOVOZYMES PRESENTATION Good Work Practices 2 Wear personal protection when needed (exposure can’t be eliminated) Keep high personal hygiene standard Wash hands often Change work clothes frequently A shower is recommended before going home

4/17/2017 NOVOZYMES PRESENTATION Good Work Practices 3 Good work practices are of special importance around high risk operations Addition of enzymes to the process, i.e. spray delivery systems Mixing/Blending Handling empty containers Maintenance of ventilation systems

Air and Medical Monitoring 4/17/2017 NOVOZYMES PRESENTATION Air and Medical Monitoring Air sampling is conducted to asses the levels of enzyme aerosols (dusts or mists) Looking for levels to be under 60ng/m3 Utilize high-volume air samplers to collect aerosols on a filter for analysis High levels may result in changes to equipment, engineering controls, or PPE for exposed employees Medical monitoring looks for early signs of problems Includes skin prick/RAST testing and lung function testing

SUMMARY Enzymes are NOT toxic. 17/04/2017 NOVOZYMES PRESENTATION Supergraphic Use: To avoid static communication the supergraphic should be placed off-center and communicate a sense of movement and evolution through its positioning. The supergraphic should always be on top of all graphic elements (i.e. dimensional planes, textures, images) except text in PowerPoint. guide SUMMARY Enzymes are NOT toxic. Like other proteins (molds, pollen, animal etc..) enzymes may cause allergic reactions in susceptible persons. Allergy symptoms occur in susceptible persons via inhalation of fine air-borne enzyme dust (< 50 micron). Irritation (eyes, skin, mucous membranes) is induced by protease only.

Safety First! Primary Safety Goal: 17/04/2017 NOVOZYMES PRESENTATION Safety First! Primary Safety Goal: Avoid generation and inhalation of dust or aerosols This can be achieved by: Enzyme product design Engineering controls Good Work Practices including Personal Protective Equipment (PPE)

17/04/2017 NOVOZYMES PRESENTATION THANK YOU!