RAPID ALCOHOL TESTING IN WHOLE BLOOD BY DISK-BASED REAL-TIME ABSORPTION MEASUREMENT J. Steigert, L. Riegger, M. Grumann, T. Brenner, J. Harter, R. Zengerle,

Slides:

Advertisements

Similar presentations

Dr. Thaweesak Tirawatnapong Chula Medical Research Center (Chula MRC)

Advertisements

Palermo – April 26, 2014 Controlling the quality of diagnostic tests E. Bravi.

Pilot Implementation of Point-Of-Care CD4 Counting in Mozambique’s National Health System Ilesh V. Jani Instituto Nacional de Saúde Maputo, Mozambique.

Using Fluorescence Spectroscopy to Determine the Relative Chlorophyll Concentrations of Vegetables Jane Kim & Rebecca Long Ch251B – Instrumental Analysis.

TEMPLATE DESIGN © High-Throughput Non-Contact Acoustic Liquid Dispensing Systems New Challenges in Automation and Implementation.

Design and Simulation of a MEMS Piezoelectric Micropump Alarbi Elhashmi, Salah Al-Zghoul, Advisor: Prof. Xingguo Xiong Department of Biomedical Engineering,

Class 7 Microfluidic Platforms Compared, Winter 2011.

MANIPULATION OF WHOLE BLOOD USING TRAVELING WAVE DIELECTROPHORESIS Y.J. Lo, A.M. Wo, and U. Lei Institute of Applied Mechanics, National Taiwan University,

On Diagnosis of Multiple Faults Using Compacted Responses Jing Ye 1,2, Yu Hu 1, and Xiaowei Li 1 1 Key Laboratory of Computer System and Architecture Institute.

MICROFLUIDIC PUMPS TO BE PRESENTED BY UMAR ABDULLAHI ABDULHAMEED MAY,2013.

Lab-on-a-chip Devices for Cell Separation and Identification A PhD project at Macquarie University under the guidance of Dr. David Inglis and Prof. Ewa.

MONOLITHIC 3-D MICROFLUIDIC DEVICE FOR CELL ASSAY WITH AN INTEGRATED COMBINATORIAL MIXER 陳睿鈞 Mike C. Liu, Dean Ho, Yu-Chong Tai Department of Bioengineering,

Enhanced light trapping in thin-film solar cells by a directionally selective filter 21 June 2010 / Vol. 18, No. 102 / OPTICS EXPRESS Carolin Ulbrich,

Sensing and Actuation in Miniaturized Systems presentation#3 Chia-Min Lin 2007/12/26 Parylene-based pressure sensor for IOP measurement Presenter : Chia-Min.

Sensing and Actuation in Miniaturized Systems presentation#2 Chia-Min Lin 2007/11/21 Parylene microvalve Presenter : Chia-Min Lin ( )

Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography EECE 491C Unger et al. (Quake group, CALTECH) Science, 2000.

SURF-IT Fellow: Bryce Kubo Mentor: Professor Marc Madou

School of Chemistry and Biochemistry

Masashi lkeuchi and koji lkuta

Photodynamic perforation of cell membrane on micro channel array toward intracellular technology Advisor ： Cheng-Hsien Liu Reporter ： Yu-Sheng Lin Date.

A CENTRIFUGATION-ENHANCED HIGH-EFFICIENCY MICRO-FILTER WITH SPIRAL CHANNEL Xu Ji, Wei Wang, Xia Lou, Jianhong Peng, and Zhihong Li National Key Laboratory.

Time-multiplexed droplet manipulation via vibrating ratcheted micro-channels IEEE MEMS(2007) Zhenwen Ding, Woo-Bin Song,Babak Ziaie Purdue University,

Professor: Cheng-Hsien, Liu Student: Hao-Ran, Shih ( ) Date: 2010/12/28 Philip N. Duncan, Transon V. Nguyen and Elliot E. Hui Department of Biomedical.

BOHB APCCB LABORATORY USE OF THE ABBOTT MEDISENSE METER FOR BETA-HYDROXYBUTYRATE MEASUREMENT. GRD Jones, A Screnci, P Graham Chemical Pathology,

CardioChek® PA Analyzer and PTS Panels® Glucose Test Strips (Reflectance) CardioChek PA Analyzer Accuracy and Precision Glucose Testing.

Concentration Measurements of Porphyrin Solutions using the Cavity Ring-Down and Integrated Cavity Output Spectroscopy Techniques Deirdre O’Leary PY4060.

Isolation of Rare Circulating Tumor Cells Sunitha Nagrath et al. Presentation.

ATWARM Advanced Technologies for Water Resource Management Marie Curie Initial Training Network Next Generation Autonomous Analytical Platforms for Remote.

Ilesh V. Jani, MD PhD Instituto Nacional de Saúde Maputo, Mozambique.

NOVELLO E., MION M.M., ZANINOTTO M., PLEBANI M. Department of Laboratory Medicine, University-Hospital, Padova (Italy). AQT90 FLEX is a fully automatic.

SCHOOL OF ENGINEERING Detection of Pathogens Using Electrochemical DNA Sensors for Resource-Limited Settings Sarah Ghanbari, Nicholas Giustini, Cameron.

Lienhard Pagel Flow injection analysis realized using PCBs S. Gassmann, L. Pagel Faculty of computer since and electronics Institute of Electronic.

Emily Salmon Norman Purvis, PhD, Esoterix Development, Simulation and Optimization of Oncology Laboratory Sample Processing Automation.

Low-Cost Microfluidic Single-Use and On-Board Reagent Storage Using Laser-Printer Technology Shau-Chieh Wang ( 王少傑 ) NTHU / MSE -- January 5,

Automatic Synthesis of Microfluidic Large Scale Integration Chips from a Domain-Specific Language Jeffrey McDaniel, Christopher Curtis, Philip Brisk University.

Si-based On-chip Optical Interconnects

CLS 541-Advanced Medical Haematology M. sc. Program Lab-2-

Engr College of Engineering Engineering Education Innovation Center Engr 1182 Nano Pre-Lab Intro to Lab-on-a-chip Rev: 20XXMMDD, InitialsPresentation.

Objectives Division of Work Background Mirror Fabrication Cavity Fabrication Biomaterials Sample Preparation Test Procedure Results Timeline Future Work.

Dual Winding Method of a BLDC Motor for Large Starting Torque and High Speed IEEE TRANSACTIONS ON MAGNETICS, VOL. 41, NO. 10, OCTOBER 2005 G. H. Jang and.

BIOPARTICLE SEPARATION AND MANIPULATION USING DIELECTROPHORESIS Advisor: Yi-Chu Hsu Student: Le Van Cong ( 黎文功 ) Date: 11/04/2011.

WHOLE BLOOD PLASMAPHERESIS USING ACOUSTIC SEPARATION CHIPS Author ： Andreas Nilsson, Filip Petersson and Thomas Laurell Dept. of Electrical Measurements,

A Wireless Bio-MEMS Sensor for C-Reactive Protein/ECG Detection Based on Nanomechanics Long-Sun Huang and Shey-shi Lu Y. H. Wang* and N. K. Chou* National.

M. J. Levene, W.W. Webb et al. Science (2003).

January 27, Paul Contestable Principal Scientist Ortho Clinical Diagnostics Second WHO Consultation: Development of a WHO Reference Panel for the.

 Oxygen Analyzer Jay Patel #19 April 27,  Senior Design Project [1]

Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of blood Jenny Zhou and Anne Ye Fan, Vermesh, et.

More efficient paper device using PNIPAM 김영완 장욱일 장수영 이원진.

BITS Pilani, Dubai Campus TECHNOFEST 2013 PAPER PRESENTATION Title of Paper Paper Area / Discipline: Students ID & Name: Date of Presentation:

Integrated Microfluidic Systems for Automatic Glucose Sensing and Insulin Injection 1 Chao-June Huang, 2 Chih-Hao Wang, 3 Yi-Hsin Chen, 3 Tse-Chuan Chou.

Continuous and Regulated Organic Micro Bubble Generation Using Lumped Gas and Organic Injected Junction Takahiro Yamamoto, Takahiro Arakawa and Shuichi.

Lab-on-a-Chip The Ideal Technology for Bio-chemical Analysis

MICROMACHINING TECHNOLOGY. Introduction Micromachining is used to create parts of size in the order of meters. Materials on a micrometer-scale possess.

Microfluidics & The Field of Bioengineering

WORKSHOP ON LABORATORY REPORT WRITING

Studies of some properties of Hydroxide-Catalysis Bonds

EVALUATION OF HEMOCHROMA POC Hb READER

Paper Microfluidic Devices

Microfluidics Roadmap for the Life Sciences

photopatterned SU-8 layers

F. v. Stetten1,2, S. Haeberle1, D. Mark1, R. Zengerle1,2, J. Ducrée1

Measurement of Analytes

Molding PDMS Channels and an Embedded Detector Chamber

Differential diagnosis of fever in West- and East-Africa

Quantitative Phase Imaging Device

Microtechnologies for biodetection and diagnostics

دانشگاه شهیدرجایی تهران

تعهدات مشتری در کنوانسیون بیع بین المللی

Figure 3 Single-cell analysis of antimicrobial susceptibility

6- 2 Testing for Intoxication

Presentation transcript:

RAPID ALCOHOL TESTING IN WHOLE BLOOD BY DISK-BASED REAL-TIME ABSORPTION MEASUREMENT J. Steigert, L. Riegger, M. Grumann, T. Brenner, J. Harter, R. Zengerle, and J. Ducr é e IMTEK – University of Freiburg, Laboratory for MEMS Applications Georges-Koehler-Allee 106, D Freiburg, Germany (μTAS2005 P.1~3) Reporter: Chung-Kuang Chin( 秦重光 ) Date:11/28/2007

Outline Introduction Design & Theory Results Conclusion

Introduction routines in clinical diagnostics (resource consumption) －＞ compact point-of-care devices －＞ lab-on-a-chip (Bio-MEMS) disk-based, colorimetric alcohol assay & optical beam-guidance integrated system to rapidly determine the concentration of alcohol in human whole blood Lab on a disk －＞

Design & Theory (1) Disk-based platform － various microfluidic functions － polymer (COC) disk (bio-compatible) － high-throughput screening (HTS) Ref:

Design & Theory (2) Optical path setup － total internal reflection (TIR) － extend optical path ( increase absorption )

Design & Theory (2) Total internal reflection (TIR)

Design & Theory (2) extend optical path － law of Beer-Lambert

Design & Theory (3) ☆ ALCOHOL ASSAY Vs = 500 nl Vr = 99.5 μl C.V. (sample metering) = 1.7 %

Design & Theory (3) ☆ ALCOHOL ASSAY

Results The performance is comparable to common breath analyzers

Conclusion This paper introduced a highly process integrated disk- based assay to rapidly and accurately determine the concentration of alcohol in human whole blood The assay is run on a cost efficient modular platform comprising a passive polymer disk with monolithically embedded optical and fluidic structures as well as a standard laser diode and detector Its high degree of automation, its short time-to-result and its excellent precision recommend our centrifugal platform for point-of-care testing and routine screening in clinical labs

THE END Thanks for Your Attention!

Reference M. Grumann et al., Proc. of MEMS 2005 conf., pp. 108–112, (2005) Jitae Kim,a Seh Hee Jang,b Guangyao Jia,a Jim V. Zoval,a Nancy A. Da Silvab and Marc J. Madou*a, Cell lysis on a microfluidic CD (compact disc),Lab on a chip, pp , (2004)