Presentation on theme: "Dr. Thaweesak Tirawatnapong Chula Medical Research Center (Chula MRC)"— Presentation transcript:
1 Dr. Thaweesak Tirawatnapong Chula Medical Research Center (Chula MRC) New Technologies and Innovations in Laboratory Support of HIV/AIDS care and TreatmentDr. Thaweesak TirawatnapongChula Medical Research Center (Chula MRC)
2 Diagnostics Tests for HIV 1 Tests to facilitate initial diagnosis2 Tests to stage the patients3 Tests to monitor the patients
3 Important of HIV Testing Known HIV status for care and treatmentFor prevention measureProblemPoor HIV testing coverage in resource- limited Setting
4 General Community Requirement Need to increase the level of access to high quality diagnostic in resource-limited settingsTo facilitate early detection and treatment of HIV/AIDSSimplify and improve the efficiency of HIV testingTests design to be delivered at The point of care (POC) or Near POC
5 The Various PCR Process have Three Steps in Common 1. Sample preparation, including viral concentration and nucleic acid extraction.2. Amplification of the target DNA or RNA.3. Detection of the amplified product.
12 New Technologies for Viral Load Testing Alere NAT systemdisposable cartridge that provides for sample collection, cell lysis, specific target capture, reverse transcription, polymerase chain reaction amplification and real time fluorescence detection based on reporter probe hybridization on an integrated micro probe array.
16 The Liat™ Analyzer is developed for nucleic acid based testing and the Liat™ Tubes serve both as collection devices and as test chambers, maintaining samples in a closed system from sample collection through sample disposal. We believe that the Liat™ system is suitable for a wide range of testing labs and near-patient test settings due to its ease of use, fast turnaround time, cost effectiveness and safety features.
46 HybridizationNanoString’s Technology employs two ~50 base probes per mRNA that hybridize in solution. The Reporter Probe carries the signal; the Capture Probe allows the complex to be immobilized for data collection
47 Purification and Immobilization After hybridization, the excess probes are removed and the probe/target complexes aligned and immobilized in the nCounter Cartridge.
48 Data CollectionSample Cartridges are placed in the Digital Analyzer for data collection. Color codes on the surface of the cartridge are counted and tabulated for each target molecule
49 nCounter Analysis System High level of precisionHigh level of sensitivity (<1 copy per cell)No enzyme processing or AmplificationQuantification
50 Multiplexed Diagnostic LLNL Awarded $2.5M to Move Pathogen Detection Tests to NanoString PlatformJune 07, 2011The National Institutes of Health has awarded $2.5 million to researchers at Lawrence Livermore National Laboratory to support the development of pathogen detection assays on NanoString Technologies' nCounter system.The project aims to develop high-content, multiplex assays capable of simultaneously detecting 35 National Institute of Allergy and Infectious Diseases category A, B, and C viral agents, plus quantifying cytokine and chemokine responses in patients,