2. Objectives Division of Work Background Mirror Fabrication Cavity Fabrication Biomaterials Sample Preparation Test Procedure Results Timeline Future Work Conclusion

3. To design and build a proof of concept experiment for a novel high sensitivity biosensor based on a Fabry-Perot cavity

4. Division of Work Initial Design Simulations and Evaluation o Sims Approach Research and Evaluation o MS Excel Biomaterial Sample Prep Fabricate device for testing o Obtain materials o Assemble the prototypes Build test setup  Write test programs to acquire data ◦ Control instruments ◦ Demodulate the signal ◦ Graph results ◦ Automate the testing/graphing procedure Rui: Jarrod: Emile:

5. Important Properties of a F-P Cavity: Free Spectral Range: Finesse: Resonant Condition: where m=1,2,3 … R=.3 R=.5 R=.9 λmλm λ m+1 λ m-1 Background

6. Glass Mirrors Glass Air Glass Air BIO Material

7. Glass Air Mirror BIO Material Glass Air Mirror

8. Mirror Fabrication Mirrors will be formed using AJA 7000 Sputtering System Many metal coatings available Au, Ag, Al, Ti, Ni, Cr Low pressure for high quality films Precise control of the thickness

9. Mirror Fabrication GlassTitaniumGold

10. Mirror Testing Transmission @ 1.55µm Glass: 87% 10Å Ti / 40Å Au: 47% 10Å Ti / 70Å Au: 18% 10Å Ti / 40Å Au 10Å Ti / 70Å Au

11. Cavity Fabrication

14. Verification of Cavity

15. Cavity Fabrication Reason for Failure: Only 150Å of gold Missing the “sticking layer” Solution: Use a sticking layer Can be Ni, Ti, Cr

16. Biomaterials in cavities: Protein G IgG Fab Same Concentration for all experimental setups

18. Coating Biomaterials GlassProtein G IgG GlassProtein G IgG Gold

20. PC/Matlab GPIB/IEEE Cable Micron Optics si720 Optical Sensing Analyzer Test Setup Single Mode Fiber Testing Block Diagram Power Input Detected light

21. Start Folder/File location to save data Start/Stop Wavelengths Figure

22. Run Matlab GUI Locate File/Folder Location to save data Start/Stop Wavelengths Start -Set the Start/Stop Wavelengths on Optical Analyzer -Start Sweeping through wavelengths -Get data results and save them in specified folder Show Results

27. 30-Jan30-Jan 6- Feb 13- Feb 20- Feb 27- Feb 5- Mar 12- Mar 19- Mar 26- Mar 2- Apr 9- Apr 16- Apr 23- Apr 30- Apr 7- May 14- May Literature Review Simulations Prepare for Report I and Presentartion Procure Raw Materials Form Mirrors and Assemble Cavity Write Code for Testing Build Test Set up and Preform Experiments Prepare for Report II and Presentation Data Analysis / Retest Prepare Final Report and Presentation

28. Future Work 1. Multi-channel Fabry-Perot cavity waveguide structure. Silicon Substrate Silicon Waveguide Multiple Channels Mirrored Ends Silicon substrate

29. Future Work 1. Multi-channel Fabry-Perot cavity waveguide structure 2. Integrated with microfluidics Biomaterials Fluid Cavity Polymer Cap

30. Waveguide with microfluidics Benefits: Reduced device size Can create more channels for biomaterials Enables mass production Uniform device size Biomaterials

31. Fabrication of Cavities is feasible Goal has been reached Introduce mirror reflectivity as a variable when testing Conclusion