BIO 344 Cell and Molecular Biology

Course description : Organization and function of biological molecules, cell diversity, cell structure and function, and cell interaction.

Prerequisites:BIO 181, CHM 152, BIO 220 or BIO 205

Egbert Schwartz Biology Building room

Lectures: Tuesday and Thursday; 9:35 a.m. – 10:50 p.m. room 265 Biology building

Office Hours Monday 12-1 Thursday By appointment Walk-in

Molecular Biology of the Cell, 2002, 4 th or 5 th edition. Alberts, Johnson, Lewis, Raff, Roberts and Walter.Garland Science, New York, NY. BOOK (required)

Web Page:

Exams: There will be three exams and a final. The final will cover all material from the class. You may elect to not take the final if you are happy with the grades on your exams

Grading: if you don’t take the final Exam I33 % Exam II33 % Exam III33 % A = B = C = D=

Grading: if do take the final Exam I20 % Exam II20 % Exam III20 % Final40% A = B = C = D=

If the final exam lowers your overall grade it will not be counted in calculating your final grade. Thus you can’t lose by taking the final.

Makeup tests You will need to make special arrangements with me if you want to take the test at another time. You will need a valid excuse such as a medical emergency or religious holiday. Arrangements must be made prior to the exam.

Attendance Attendance for this class is not required. However, because most questions will come directly from the lectures, the probability of receiving a decent grade without attendance is very small. Recognize that the book is very difficult and part of the book will not be on the exam

Brief preview of lectures chapter 10 – 13, 15-19

Membrane structure

Membrane Transport

Protein sorting

Vesicular transport

Cell to cell communication

Cytoskeleton

Cell Cycle and Cell Death

Cell Division

Cell Junctions and Adhesion

The response of blood platelets to injury

Platelets prior to activation

Ca 2+ and diacylglycerol activate protein kinase C

Actin filament dynamics and platelet activation in

Actin filament dynamics and platelet activation

Activated platelet with large spread lamellipodium

Activated platelet after contraction

Chapter 10 Membrane Structure

4.7 – Part 1 figure 04-07a.jpg 1

Eukaryotic Cell

The plasma membrane is a lipid bi-layer 2

2-dimensional model of plasma membrane

3-dimensional model of plasma membrane

Phosphatidylcholine 3

4

Polar molecule dissolved in water Arrangement of water molecules is barely disturbed 5

Non-polar molecule dissolved in water Arrangement of water molecules strongly disturbed

Shape of lipid determines packing of lipid molecules

Figure 10-11a Molecular Biology of the Cell (© Garland Science 2008)

Formation of sphere is energetically favorable

Figure 10-9a Molecular Biology of the Cell (© Garland Science 2008)

Figure 10-9b Molecular Biology of the Cell (© Garland Science 2008)

Figure 10-11b Molecular Biology of the Cell (© Garland Science 2008)

Figure Molecular Biology of the Cell (© Garland Science 2008)

Figure Molecular Biology of the Cell (© Garland Science 2008) Effect of cis double bonds on membranes

Figure Molecular Biology of the Cell (© Garland Science 2008) 1:1 phosphotidylcholine and sphingomyelin 1:1:1 phosphotidylcholine and sphingomyelin and cholesterol

Figure 10-14b Molecular Biology of the Cell (© Garland Science 2008)

Figure 10-14a Molecular Biology of the Cell (© Garland Science 2008)

DVD clip 36 6,7

Cholesterol 9

Cholesterol in lipid bilayer

DVD Clip 37

10

Lipid composition varies among different cell types 11