King Lab Yearly Safety Review by Cammie Haase-Pettingell for March 2005 Disclaimer- This is Jonathan King’s lab in Biology Dept. yearly safety review and not to be substituted for an initial entry orientation. Any questions should be directed to Cammie

March 19, 2004CHP GM Points be covered Emergency Action Plan Eating Chemical Radioactive Biologicals, Blood Borne Pathogens, Infectious Agents High Voltage

March 19, 2004CHP GM Responsibility PI Ensure that the lab is conducting in a safe manor  This is by the PI or by a representative the safety rep. Safety Rep. To conduct incoming safety orientation Go to safety meetings Update the lab on new policy Individual To follow the above policy

March 19, 2004CHP GM Basics No Eating in lab Can eat in  Tea room  Computer room,  hall and seminar room No smoking except outside No mouth pipeting Story…

March 19, 2004CHP GM Evacuation Plan

March 19, 2004CHP GM Fire Close the lab doors Pull the fire red fire alarm Located by Stairwells, Elevators Leave by stairwells Get to a phone- dial x100 report fire Don’t hang up till they have hung up Not responsible to fight a fire

March 19, 2004CHP GM Unknown fire such as strange smell If in doubt call x 100 Pull fire alarm Other scenario Look around Call John Fuccilo to ask he know anything Ex. melted stuff in autoclave

March 19, 2004CHP GM Evacuation The equipment halls can’t hear the alarm As exit ensure people know about the alarm If can - put experiments to rest- not required you safety is more important So firefighters  ie do not stumble upon a active electrophoresis. Just in case not back for a while may not be ruined Evacuate by stairwell -all are fire stairwells Meet by the concrete post by bld 66 even if you think I saw you Check in so we know no one is stuck in the building  Then go off for what ever. Some one needs to go to the command post say we are all out by the door toward Main St. -

March 19, 2004CHP GM Chemicals MSDS see handout Chemical safety web training Spill kits D behind door under sink

March 19, 2004CHP GM Hoods Simple Laminar Flow Hood Ensure the back “slot” in back is clear have items on lifts so it is not blocked Simple test for flow to tape Kimwipe If malfunctioning- inform fixit Zone -A Rooms air flow and temperature is dependant on the hood.

March 19, 2004CHP GM Spills Chemical Radioactivity Biological General Clear Area Get on appropriate safety gear Determine the Scope Use the appropriated cleaner Dispose correctly Look at the Red flip chart by phone

March 19, 2004CHP GM Chemical Spill 1. Evacuate area- put sign up 2. Done appropriate safety - lab coat, eye protection, appropriate gloves 3. Determine the necessary sorbant, 1. For acid base or solvent. 4. Pour around the outside of the spill - mix in Hold breath 5. I use the dust pan and broom sweep up. 6. Wipe up residue 7. Dispose of as waste

March 19, 2004CHP GM Chemicals and Medical Emergency Do not block body showers Eye wash put injured eye down flush for 15 min. Eyewash or distilled wash with tubing Needs to be tempered water Cut and injury- should go to Medical can be documented and treated properly Someone collapses Ensure you are not putting you self in danger If can rise -call EMT X100 EMT will be concern that they are not in danger try to figure what happened

March 19, 2004CHP GM Remember Emergency number X100 If working alone at night inform some one on the Hall Or call campus police Safety is important to everyone.

March 19, 2004CHP GM Chemical waste- Waste needs a red tag Must be closed when not in use. Most is in electrophoresis room Silver- silver stain MEOH-  Gel drying fix., Transfer solution Ethidium bromide -  Liquid and tips  Agarose in the dark room Once full date and should be picked up immediately by law 2 days If can call don’t date till wedded in link on King links page

March 19, 2004CHP GM Sharps Two types Biological or not chemical waste-gray bin  If it is sharp from a biological lab it can not be just thrown out. Chemical waste- with red tags At the Bench Need to have covers and be covered when not in use. Labeled

March 19, 2004CHP GM Radiation ext We use 14 C and 35 S to label rapidly proteins Disposal designated by 1/2 life day 1/2 life - 35 S- 88 day 1/2 life Over 120 day 1/2 life 14 C = 5730 yr 1/2 life  Type- dry (paper gloves), hi level aqueous, sharps/epi. Radioactivity stocks is stored in the locked refrig and freezer D Most experiment are preformed in the D. Geiger counters in each side of the lab. Pancake probes more sensitive Ensure the battery is off when done.

March 19, 2004CHP GM Radioactive spill Clear the area- block off Ensure they you have lab coat, safety glasses, double gloves, boots? Monitor the area with Geiger counter Mark the area- where there are counts One does not inactivate the radioactive just clean off the solution. Good agents -Bleach, Radiact wash, Am 4 OH Clean till no signal RPO will do wipe test If still signal cover till decayed.

March 19, 2004CHP GM Biological BL1- E. Coli low BL2 - Salmonella typhurminium LT2 Clean up All bacteria needs to be in activated by before disposal or going to the kitchen  Autoclaving  Chemical, 10% bleach, 1% Westcodyne, Lysol Waste Plates go into the bucket autoclaved in big autoclave after kitchen hours.

March 19, 2004CHP GM Biological spills Block the area Let aerosol settle Safety gear Clean up by pour/spray disinfectant on glassware with dusty pan Put paper towel on it and pour/spray disinfectant on it Dispose of in the waste. Look for the new biological spill kits

March 19, 2004CHP GM Decontaminating the Bench 70% ethanol is a better agent to decontaminate the bench than 100% ethanol 70% is better at denaturing the outer membrane and subsequently lysing the cell 100 % will only causes effects the on the outer membrane but not lysis.

March 19, 2004CHP GM High Voltage Any voltage should be treated as if it is dangerous. AC current is the most dangerous. Running a PAGE Start up a gel by connecting the black lead first as a ground and then the red lead.  It should be noted that high power leads should be used when running a sequencing gel with high voltage. Turn off a power supply by turning down the voltage source, then turn off the power.  After turning off power, disconnect the red (positive) lead first. If the hazard light is flashing on the power supply, turn it off and determine what the problem is and fix it, or have it fixed. If someone is unconscious, call x100 which is the emergency phone number at MIT.

March 19, 2004CHP GM Chemical Hygiene Plan OSHA right to know MSDS know how to read SOP- Standard Operating Procedures a written procedure for use of every chemical

March 19, 2004CHP GM Safety is everyone responsibility Everyone will be responsible for the level 1 check list which I the pdf keep check off for a month Cammie and Shannon are the safety Reps to do an over view ensure all unlabeled liquid containing item have a label can be cryptic