CD68 NE-SSE NE-NS Figure E1. Nrf2 42-kD  actin 32-kD HO-1 100-kD Control si RNA si RNA Nrf2 Control si RNA si RNA Nrf2 Control si RNA si RNA Nrf2 Control.

Slides:

Advertisements

Similar presentations

Supplementary Figure 1. Caspase 8 expression and activation and Fas expression in NSCLC and normal lung cell lines. A Western blot analysis of DR4 and.

Advertisements

Control siRNA BRG1 siRNA PTEN – Akt – p-Akt – p-GSK-3  – Cyclin D1 – BRG1 –  -actin – SW480 Supplemental data S1 Supplemental data S1. Effects of transduction.

Tfam 1  g empty vector 1  g Tfb2m 1  g Relative mRNA level * * p

Knight et al – Supplementary Table Supplemental Table 1: Cell line Densitometry AROSSIRT1 ARPE WI MCF10A HCT HCT116.

P247. Figure 9-1 p248 Figure 9-2 p251 p251 Figure 9-3 p253.

Online table I: Sequences of primers used. ControlIron RM M2 I ControlIron RM M2 ML Iron *** Iron content (nmol/mg protein) **

Sh-luc shSCP1-1 * IB: c-Myc IB: Tubulin shSCP1-2 IB: p-Ser KD 70KD 60KD 70KD (abnova) sh-luc shSCP1-1 * IB: c-Myc IB: Tubulin shSCP1-2.

Supplemental Results Online Figure S1. Norepinephrine increases ROS production. (A): H9c2 cells were treated with 10  M norepinephrine and intracellular.

Figure S1 Enod11 Mtc27 MtGshs cDNA gDNA S. meliloti DPI Supporting Information Fig. S1. Validation of the selected biological conditions for.

Table S1. Primers for quantitative RT-PCR β-actinSense5'-ATCTGGCACCACACCTTC-3' Antisense5'-AGCCAGGTCCAGACGCA-3' CD36Sense5'-TGGTCAAGCCAGCTAGAAA-3' Antisense5'-CCCAGTCTCATTTAGCCAC-3'

(hr) Cleaved Caspase-9 Caspase nM Paclitaxel MCF-7 Figure S1a.

A Supplementary Figure 1. ChIP-seq Sequencing Statistics b RNA-seq Sequencing Statistics Sample NameSequencing Depth Uniquely Mapped Reads Number of peaks.

Figure S1 HA-FOXO3 wt C terminus ab HA-FOXO3 wt HA ab untransfected C terminus ab untransfected HA ab

Supplemental Digital Content 4

Fig. 4. Effect of curcumin on urine SOD and MDA, Nrf2/Keap1, and HO-1 protein expression in the urine or renal cortex. (A) Urinary SOD was significantly.

Α-Lipoic Acid Promotes Neurological Recovery After Ischemic Stroke by Activating the Nrf2/HO-1 Pathway to Attenuate Oxidative Damage Cell Physiol Biochem.

Supplemental Figure 2. (A) AtplaIVA-1 and AtplaIVA-2 null transcription lines for AtPLAIVA mRNA. RNAs from the relevant wild type Col were isolated.

Hypoxia increases IL-32 expression in an HIF1α-dependent manner.

Cytosol Nucleus Mitochondria Secreted ER Golgi Endosome Lysosome Transmembrane.

Juewon Kim, Hyunjung Choi, Eun-Gyung Cho, Tae R. Lee

Volume 34, Issue 6, Pages (June 2009)

Supplementary Figure 1 A B catalase p53 catalase MW(kDa) PIG3 MW(kDa)

* * Supplemental Figure S-I Con AS LC3/DAPI

Impaired Activation of the Nrf2-ARE Signaling Pathway Undermines H2O2-Induced Oxidative Stress Response: A Possible Mechanism for Melanocyte Degeneration.

Volume 32, Issue 5, Pages e6 (November 2017)

Volume 143, Issue 4, Pages e9 (October 2012)

This month in Gastroenterology

Aryl Hydrocarbon Receptor Is an Ozone Sensor in Human Skin

Palladium and Platinum Nanoparticles Activate AHR and NRF2 in Human Keratinocytes—Implications in Vitiligo Therapy Gaku Tsuji, Akiko Hashimoto-Hachiya,

Simvastatin Protects Human Melanocytes from H2O2-Induced Oxidative Stress by Activating Nrf2 Yuqian Chang, Shuli Li, Weinan Guo, Yuqi Yang, Weigang Zhang,

PMI: A ΔΨm Independent Pharmacological Regulator of Mitophagy

Volume 13, Issue 10, Pages (December 2015)

MicroRNA-101 Exerts Tumor-Suppressive Functions in Non-small Cell Lung Cancer through Directly Targeting Enhancer of Zeste Homolog 2 Ji-guang Zhang,

Volume 17, Issue 1, Pages (January 2013)

Proteasomal Degradation Is Transcriptionally Controlled by TCF11 via an ERAD- Dependent Feedback Loop Janos Steffen, Michael Seeger, Annett Koch, Elke.

Cellular localization of the “writers” and “erasers” in U2OS-G3BP1 cells. Cellular localization of the “writers” and “erasers” in U2OS-G3BP1 cells. (A)

Supplementary Figure S1

Volume 21, Issue 12, Pages (December 2017)

Induction of the nuclear factor (erythroid derived 2)-like 2 antioxidant response element (Nrf2-ARE) target genes in mesenchymal stromal cells (MSCs) from.

Supplementary Figure 1. A

Supplementary Figure 1 IP: IgG HA 3HA-E2F1: cIAP1: wt K

Daniela Macconi, Giuseppe Remuzzi Kidney International

Bernard et al, Supplementary Figure S1

Identification of Ketoconazole as an AhR-Nrf2 Activator in Cultured Human Keratinocytes: The Basis of Its Anti-Inflammatory Effect Gaku Tsuji, Masakazu.

Supplementary Figure S4

Volume 85, Issue 1, Pages (January 2014)

Juliana Saraiva Anjos, MD, Ludmila F. M. F

Nrf2 Promotes Keratinocyte Proliferation in Psoriasis through Up-Regulation of Keratin 6, Keratin 16, and Keratin 17 Luting Yang, Xueli Fan, Tingting.

A1 A2 A3 A4 Figure S1. (A2, C2) hPDLSCs at P2 show low expression of p16 and p21 (red fluorescent) as indicated by double immunostaining. (A3, C3) Cells.

N H N H Figure S1 parp-1 +/+ parp-1 -/- HIF-2 DAPI

Reh cells, 24 hours c-Myc Ac-p53 b-actin

Aberrant Regulation of the MRP3 Gene in Non-small Cell Lung Carcinoma

Volume 10, Issue 2, Pages (January 2015)

Knockdown of Stat3 activity in vivo prevents diabetic glomerulopathy

USP15 Negatively Regulates Nrf2 through Deubiquitination of Keap1

Nrf2−/− mice had higher TGF-β1 transcription and FN expression.

Synergistic WNT1 and WNT7B signaling is downstream of LRP6 phosphorylation and β-catenin stabilization. Synergistic WNT1 and WNT7B signaling is downstream.

Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased survivin expression. Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased.

Volume 26, Issue 12, Pages e5 (March 2019)

Fig. 7. Nrf2-dependent enzyme activities in wild-type, Nrf2- and Keap1-deficient tissues.Hepatic (A,C,E) and cortical (B,D,F) enzyme activities of NQO1.

Inhibition of polymerization Enhancement of polymerization

Volume 85, Issue 1, Pages (January 2014)

* * * ** ** ** ** ** ** Fig. 1..

Nrf2 was activated by high glucose–induced ROS production in HRMCs

A Cell type Wild type Nox4-/- CD4+ T cells CD8+ T cells CD4- CD8- NK

Role and Regulation of STAT3 Phosphorylation at Ser727 in Melanocytes and Melanoma Cells Masanobu Sakaguchi, Masahiro Oka, Tetsushi Iwasaki, Yasuo Fukami,

Effect of SFN on the protein expression of Nrf2, HO-1, and NQO1 in JB6-shMock and JB6-shNrf2 cells. Effect of SFN on the protein expression of Nrf2, HO-1,

Nested RT-PCR of Mammaglobin and CK19 mRNA in plasma and circulating cells of controls (C) and patients (T) with breast cancer. Nested RT-PCR of Mammaglobin.

Fig. 3. KMS99220 inhibits activation of NFκB signaling via HO-1 induction. (A) BV2 cells were treated with 10 µM KMS99220 for 1 h, and then treated with.

scrambled si-CLOCK scrambled si-CLOCK scrambled si-CLOCK scrambled

Presentation transcript:

CD68 NE-SSE NE-NS Figure E1

Nrf2 42-kD  actin 32-kD HO kD Control si RNA si RNA Nrf2 Control si RNA si RNA Nrf2 Control si RNA si RNA Nrf2 Control si RNA si RNA Nrf2 4h8h12h24h Figure E2 A B 4h8h12h24h si RNA Nrf2 * * Nrf2 /  -actin ratio (% vs. time matched control) * * HO-1 /  -actin ratio (% vs. time matched control) 4h8h12h24h si RNA Nrf2

Figure E3 * Cytosolic Nrf2  -actin ratio (% vs. control) Control si RNA si RNA Nrf2 8h Cytosolic Nrf2  actin 100-kD 42-kD Control si RNA si RNA Nrf2 8h Nuclear Nrf2 Lamin B1 100-kD 68-kD B A * Nuclear Nrf2 /lamin B1 ratio (% vs. control) Control si RNA si RNA Nrf2 C Nrf2HO-1 TO-PRO-3 MERGE siRNA Nrf2 300nM - 8h siRNA control 300nM - 8h Control si RNA si RNA Nrf2

* * * * * * 4h8h12h24h si RNA Nrf2 4h8h12h24h si RNA Nrf2 * * * 4h8h12h24h si RNA Nrf2 Figure E4 mRNA HO-1/ Ubiquitin (% vs. time matched control) mRNA NQO1/ Ubiquitin (% vs. time matched control) mRNA GPX2/ Ubiquitin (% vs. time matched control)