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0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a.

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Presentation on theme: "0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a."— Presentation transcript:

1 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel MCF-7 Figure S1a

2 MCF-7 Figure S1b * **

3 - - + + - - - + - + - +Paclitaxel 100 nM Bad siRNA Scrambled siRNA - - - - + + Bad Actin IP: Bcl-2 IB: Bcl-2 Input IB: Bcl-2 Bcl-2 Actin IP: Bcl-2 IB: Bim BimEL BimL BimS MCF-7 Figure S1c

4 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel Cleaved Caspase-3 T47D Figure S1d

5 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel Cleaved Caspase-3 MDA-MB-468 Figure S1e

6 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 100 nM Paclitaxel Cleaved Caspase-3 BT20 Figure S1f

7 IP: Bcl-xL IB: Bcl-xL Bcl-xL Bim EL Bim L Bim S IP: Bcl-xL IB: Bim Mcl-1 Paclitaxel 100 nM 0 4 8 12 [h] Bim EL Bim L Bim S IP: Mcl-1 IB: Bim Paclitaxel 100 nM 0 4 8 12 [h] IP: Mcl-1 IB: Mcl-1 Figure S2a MCF-7

8 Figure S2b Pre-IPPost-IP BimEL BimL BimS HM LMS-100 Untreated Taxol 12 h CoxIV BiP/Grp78 BimEL BimL BimS 1 0.43 Actin Ratio Paclitaxel 100 nM - + Pre-IPPost-IP Actin BimEL BimL BimS Untreated 1 1.39Ratio HM LMS-100 MCF-7

9 - + - + Paclitaxel 100 nM MCF-7 MCF-7 Bcl-2+ FLAG-Bcl-2 Bcl-2 FLAG-Bcl-2 BimEL BimL BimS IP: FLAG IB: Bcl-2 Input IB: Bcl-2 IP: FLAG IB: Bim Bmf IP: FLAG IB: Bmf Input IB: Bmf BimEL BimL BimS Input IB: Bim Actin IP: FLAG IB: Bax IP: FLAG IB: Puma * IP: FLAG IB: Bak IP: FLAG IB: Bad - + - + Paclitaxel 100 nM MCF-7 MCF-7 Bcl-2+ Bad Puma Bax Bak Figure S2c

10 Figure S2d IP: Bcl-2 IB: Bax Bax IP: Bcl-2 IB: Bak Paclitaxel 100 nM - + IP: Bcl-xL IB: Bax IP: Bcl-xL IB: Bak Paclitaxel 100 nM - + IP: Mcl-1 IB: Bax IP: Mcl-1 IB: Bak Paclitaxel 100 nM - + Bak Bax Bak Bax Bak MCF-7

11 0 4 8 12 24 48 0 4 8 12 24 48 (hr) MitoCyto Cytochrome c CoxIV T47D Figure S3a

12 0 4 8 12 24 48 0 4 8 12 24 48 (hr) MitoCyto MDA-MB-468 Figure S3b Cytochrome c CoxIV

13 0 4 8 12 24 48 0 4 8 12 24 48 (hr) MitoCyto BT20 Figure S3c Cytochrome c CoxIV

14 0 4 8 12 24 48 (hr) Bax Bak IP: Bax 6a7 IB: Bax N20 IP: Bak Ab-2 IB: Bak G23 100 nM Paclitaxel T47D Input IB: Bax N20 Input IB: Bak G23 Bax Bak Actin Figure S4a

15 0 4 8 12 24 48 (hr) Bax Bak IP: Bax 6a7 IB: Bax N20 IP: Bak Ab-2 IB: Bak G23 100 nM Paclitaxel MDA-MB-468 Input IB: Bax N20 Input IB: Bak G23 Bax Bak Actin Figure S4b

16 0 4 8 12 24 48 (hr) Bax Bak IP: Bax 6a7 IB: Bax N20 IP: Bak Ab-2 IB: Bak G23 100 nM Paclitaxel BT20 Input IB: Bax N20 Input IB: Bak G23 Bax Bak Actin Figure S4c

17 Puma Actin - - + + - - - + - + - +Paclitaxel 100 nM Puma siRNA Scrambled siRNA - - - - + + MDA-MB-468 Figure S5a

18 Puma Actin - - + + - - - + - + - +Paclitaxel 100 nM Puma siRNA Scrambled siRNA - - - - + + BT20 Figure S5b

19 Puma Actin - - + + - - - + - + - +Paclitaxel 100 nM Puma siRNA Scrambled siRNA - - - - + + MCF-7 Figure S5c

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