1. Figure S1 HA-FOXO3 wt C terminus ab HA-FOXO3 wt HA ab untransfected C terminus ab untransfected HA ab 5.9 6.7 6.4

2. Figure S2 A FOXO3 IP Modification ab detect Modification ab IP FOXO3 ab detect cIEF IP pSer/Thr ab Detection FOXO3 ab IP Acetyl-K ab Detection FOXO3 ab IP Methyl-R ab Detection FOXO3 ab IP Ubiquitin ab Detection FOXO3 ab IP FOXO3 ab Detection Methyl-R ab IP FOXO3 ab Detection Acetyl-K ab IP FOXO3 ab Detection Ubiquitin ab Detection FOXO3 pSer 253 ab Detection FOXO3 pSer318/321 ab UT HCV UT HCV C B UT HCV UT HCV UT HCV UT HCV UT HCV UT HCV

3. Modification ab detect FOXO3 ab detect cIEF FOXO3 IP Figure S3 IP FOXO3 ab Detection pSer/Thr ab IP FOXO3 ab Detection FOXO3 ab IP FOXO3 ab Detection MeR ab IP FOXO3 ab Detection AcLys ab IP FOXO3 ab Detection Ubiquitin ab UT HCV EtOH EtOH/ HCV UT HCV EtOH EtOH/ HCV UT HCV UT HCV EtOH UT HCV EtOH EtOH/ HCV EtOH EtOH/ HCV AB

4. Figure S4 A B C Relative luciferase activity D HA FOXO3 S294A HA FOXO3 S425A HA FOXO3 S574A overexpressed wt FOXO3 untreated JFH1 JNK inhibitor JFH1 + JNK inhibitor RFP reporter E Relative protein FOXO3 in the nucleus ** 5.95 5.78 5.92 5.93

5. Figure S5 A B

6. Figure S6 FHRE Luciferase Relative mRNA N C N C N C - - + + + + EtOH - + - + - + JFH1 - - - - + + SAM FOXO3 actin C * D * ** Relative luciferase activity AB

7. overexpressed wt FOXO3 untreated JFH1 EtOH EtOH JFH1 Figure S7 PRMT1 FOXO3 actin CNCN Control PRMT1 siRNA A C Load UT WT R_K HA IP FOXO3 14-3-3 B

8. A B PRMT1 Actin N C N C UT JFH1 EtOH E+J MeR GAPDH UT JFH1 EtOH E+J ** Figure S8 actin PP2A UT EtOH JFH1 E+J C