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Tfam 1  g empty vector 1  g Tfb2m 1  g Relative mRNA level 0 100 200 50 150 * * p<0.05 vs empty vector * Transfection Tfam mRNA Tfb2m mRNA Tfam and.

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Presentation on theme: "Tfam 1  g empty vector 1  g Tfb2m 1  g Relative mRNA level 0 100 200 50 150 * * p<0.05 vs empty vector * Transfection Tfam mRNA Tfb2m mRNA Tfam and."— Presentation transcript:

1 Tfam 1  g empty vector 1  g Tfb2m 1  g Relative mRNA level 0 100 200 50 150 * * p<0.05 vs empty vector * Transfection Tfam mRNA Tfb2m mRNA Tfam and Tfb2m mRNA Tfam siRNA #1 Scramble siRNA Tfb2m siRNA #1 0 0.75 0.5 0.25 1 * * Tfam siRNA #2 Tfb2m siRNA #2 * * p<0.05 vs scramble siRNA * Tfam mRNA Tfb2m mRNA Transfection Tfam and Tfb2m mRNA Tfam Tfb2m 28S Non-treated Tfam siRNA #1 Scramble siRNA Tfb2m siRNA #1 Tfam siRNA #2 Tfb2m siRNA #2 Relative mRNA level Supplemental Figure 2 B C A Transfection

2 A Supplemental Figure 3 Translational diffusion time (  s) 0 200 400 600 800 1000 1200 1400 -1769-1739-1709-1679-1649-1619-1589-1559-1529-1499-1469-1439-1409 p<0.05 * -1799-1829 Recombinant TFAM protein (-) (+) -1379 * B Translational diffusion time (  s) 0 200 400 600 800 1000 1200 1400 -1769-1739-1709-1679-1649-1619-1589-1559-1529-1499-1469-1439-1409 *p<0.05 * -1799-1829 Recombinant TFB2M protein (-) (+) -1379 * 1600 1800 * *

3 Supplemental Figure 4 A B Tfam siRNA #1 Scramble siRNA 0 15 10 5 20 * Tfb2m siRNA #1 * p<0.05 vs. scramble siRNA * ATP content (nmol/mg tissue) 0 30 20 10 40 * * p<0.05 vs. empty vector * ATP content (nmol/mg tissue) Tfam 1  g Empty vector 1  g Tfb2m 1  g

4 Supplemental Figure 5 AB Relative mitochondrial ATPase 6/8 mRNA level r = 0.49 p < 0.01 0.25 0.5 0.75 1.0 1.25 2.02.51.51.00.5 Relative Tfam mRNA level 1.5 1.75 2.0 Relative mitochondrial ATPase 6/8 mRNA level r = 0.57 p < 0.001 0.25 0.5 0.75 1.0 1.25 2.01.51.00.5 Relative Tfb2m mRNA level 1.5 1.75 2.0 D Relative cytochrome-b mRNA level r = 0.48 p < 0.01 0.25 0.5 0.75 1.0 1.25 2.02.51.51.00.5 Relative Tfam mRNA level 1.5 C Relative cytochrome-b mRNA level r = 0.58 p < 0.001 0.25 0.5 0.75 1.0 1.25 2.01.51.00.5 Relative Tfb2m mRNA level 1.5

5 Translational diffusion time (  s) -170 ~-141 -164 ~-135 -158 ~-129 -152 ~-123 -146 ~-117 -140 ~-111 -134 ~-105 -131 ~-102 -128 ~-99 -125 ~-96 -122 ~-93 ( - ) (+)(+) p<0.05 0 500 1000 1500 2000 2500 3000 * * * * * Recombinant SP1 protein A CD Tfam 0.5  g + Sp1 0.5  g 1 2 0.5 1.5 2.5 0 Empty vector 1  g Tfam 1  g Sp1 1  g 3 Serca2 gene transcription (relative luciferase unit) * * * p<0.05 vs. empty vector 0 200 400 600 800 1000 1200 TFAM only TFAM+SP1 Translational diffusion time (  s) NS Supplemental Figure 6 B Serca2 gene transcription (relative luciferase unit) p<0.05 vs. empty vector in each group * * * Serca2 Luc wild Serca2 Luc mutation -122 Serca2 Luc mutation -119 Empty Tfam Tfb2m Sp1 Empty Tfam Tfb2m Sp1 Empty Tfam Tfb2m Sp1 Empty Tfam Tfb2m Sp1 Serca2 Luc mutation -116 * * * 0 1 2 3 4 * * * *

6 Tfam Tfb2m Bnp Serca2a ET-1 100 nmol/L NE 30  mol/L NE 100  mol/L Vehicle 28S Supplemental Figure 7 ET-1 10 nmol/L Vehicle NE 5  mol/L Vehicle

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