MYC is required for the hypoxic induction of SHMT2.

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MYC is required for the hypoxic induction of SHMT2. MYC is required for the hypoxic induction of SHMT2. A, SH-SY5Y, Kelly, SK-N-BE(2), and SF188 cells were exposed to 0.5% O2 for 6 or 16 hours. Left, mRNA levels were measured using qPCR and normalized to normoxia control. Right, immunoblots of N-MYC and c-MYC in the four cell lines. B, SF188 cells were transfected with MYC siRNA and exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 8 (for qPCR) or 16 hours (for immunoblot); mRNA levels were measured using qPCR (left). Protein levels of HIF1α, MYC, and SHMT2 were examined by immunoblot (right). C, SH-SY5Y cells were transfected with 2 μg pcDNA–c-MYC or vector control (VC). After 48 hours, transfected cells were exposed to normoxia (Nor) or 0.5% O2 (Hyp) for 8 hours. mRNA levels were measured using qPCR (left). c-MYC overexpression was confirmed using immunoblot (right). A to C, data represent mean ± SD of triplicate PCR reactions, and representative of three independent experiments is shown. *, P < 0.05; **, P < 0.01; ***, P < 0.001; determined by the Student two-tailed t test. Jiangbin Ye et al. Cancer Discovery 2014;4:1406-1417 ©2014 by American Association for Cancer Research