1. RPL26 is a positive regulator of TP53β splicing.
RPL26 is a positive regulator of TP53β splicing. A, p53β splicing in the absence of RPL26. Left, relative amount of TP53β (TP53β mRNA/TP53 pre-mRNA) in MCF-7 cells transfected with two different RPL26 siRNAs (L26siA and L26siB) for 3 days before 10 μmol/L CP or DMSO treatment for 8 hours. Right, RT-qPCR analysis of nuclear mRNA levels in MCF-7 cells with the same treatment as described above. ***, P ≤ 0.001, t test. B, Enrichment of TP53 pre-mRNA on nuclear RPL26 in the absence of SMG1. The bound mRNAs in nuclear RPL26 immunoprecipitated from MCF-7 cells transfected with SMG1 siRNA for 3 days were subjected to RT-qPCR analysis. Left, mRNA levels detected in RPL26 immunoprecipitate from nuclear fraction. Right, mRNA expression in input nuclear fraction. Shown are mean ± SEM of three independent experiments. **, P ≤ 0.01, t test.
Jing Chen et al. Cancer Discov 2017;7:
©2017 by American Association for Cancer Research