1. KIF1Bβ promotes DHX9 nuclear localization.
KIF1Bβ promotes DHX9 nuclear localization. A, left, anti-KIF1Bβ immunoblot analysis of NB1 and SK-N-SH cell lines. Right, immunofluorescence images of NB1 and SK-N-SH cells stained for DHX9 (green) and counterstained with Hoechst to visualize nuclei (blue). B, fluorescence studies of NB1 and SK-N-SH cells 24 hours after transfection with plasmid encoding eCFP-DHX9 (green) as indicated. C, fluorescence studies in NB1 cells 24 hours after transfection with plasmid encoding eCFP-DHX9 together with either RFP-KIF1Bβ-WT or RFP-KIF1Bβ mutants as indicated (arrow). D, graphical representation showing percentage of transfected cells with nuclear or cytoplasmic eCFP-DHX9 localization (mean ± SD; n = 3; **, P < 0.01; ***, P < 0.001). E, graphical representation of immunofluorescence images of NB1 cells (Supplementary Fig. S3D) transfected with Flag-KIF1Bβ mutants as indicated. Shown is the percentage of nuclear or cytoplasmic eCFP-DHX9 or both 24 hours after transfection (mean ± SD; n = 3; *, P < 0.05; **, P < 0.01; ***, P < 0.001). F, anti-DHX9 (green) immunofluorescence staining of NB1 cells 24 hours after transfection with plasmids encoding Flag-KIF1Bβ wild-type (WT) or disease-causing variants as indicated (red). The percentage of transfected cells displaying nuclear DHX9 is shown in G (mean ± SD; n = 3; *, P < 0.05; **, P < 0.01; ***, P < 0.001).
Zhi Xiong Chen et al. Cancer Discovery 2014;4:
©2014 by American Association for Cancer Research