1. MYC degradation screen identifies a compound that stabilizes MYC protein.
MYC degradation screen identifies a compound that stabilizes MYC protein. (A) GPS-MYC cells were treated with 20 μM UNC for 6 hours, and EGFP and DsRed intensities were measured by flow cytometry. Data are from the GPS-MYC screen. (B and C) GPS-MYC cells were treated with increasing concentrations of UNC for 6 hours and EGFP and DsRed intensities were measured by flow cytometry (B) or immunoblotting (C). (D) KRAS-mutant PDAC cell lines were treated for 6 hours with UNC , and MYC protein abundance was measured by immunoblotting (top), with quantitation by densitometry relative to vehicle control (bottom). *P < 0.05, **P < 0.01, ***P < by t test. (E) MIA PaCa-2 cells were treated for 6 hours with UNC , and MYC mRNA expression was measured by quantitative PCR (qPCR). MYC mRNA expression was normalized to that of GAPDH mRNA. a.u., arbitrary units. (F) Kinase selectivity of UNC as described previously (30). (G) MIA PaCa-2 cells were treated for 6 hours with the indicated compounds, and MYC protein abundance was measured by immunoblot. All data are representative of at least three independent experiments. Data in (D) are presented as means ± SD.
Devon R. Blake et al., Sci. Signal. 2019;12:eaav7259
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