Looking at periodicity in protein sequence and structure

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Presentation transcript:

Looking at periodicity in protein sequence and structure

The enzyme adenylosuccinate lyase

The enzyme adenylosuccinate lyase

The enzyme adenylosuccinate lyase Sequence of one of the surface helices: LLIRRALAAVKEKARAVGDQLASM Is this sequence amphipathic when examined in an a-helical configuration?

The enzyme adenylosuccinate lyase LLIRRALAAVKEKARAVGDQLASM LLIRQAISLVKEKAKAVGEELTRL LLVRRALGHIKRKVNAVGGELASL LLIRRALSIIKKKINAAGEELARL LLIRRALSIVKRKLDIAGEELKRL IIVKKALEIVKKKLRDVITILIEY LVFRDAIRLVKTKVVEILEMMVSL LIFRDAIRLVKRKIMEILEIMISL LIIRDALAKVYKDLKEIIKKFLEI LVLREALSIIYRRLHRLAEVLAEK 234251255325346444563655 Number of different amino acids types at the given position In a multiple sequence alignment, does the degree of sequence variation at different positions reflect a potential alpha helical character?

The enzyme adenylosiccinate lyase LLIRRALAAVKEKARAVGDQLASM LLIRQAISLVKEKAKAVGEELTRL LLVRRALGHIKRKVNAVGGELASL LLIRRALSIIKKKINAAGEELARL LLIRRALSIVKRKLDIAGEELKRL IIVKKALEIVKKKLRDVITILIEY LVFRDAIRLVKTKVVEILEMMVSL LIFRDAIRLVKRKIMEILEIMISL LIIRDALAKVYKDLKEIIKKFLEI LVLREALSIIYRRLHRLAEVLAEK 234251255325346444563655 Number of different amino acids types at the given position In a multiple sequence alignment, does the degree of sequence variation at different positions reflect a potential alpha helical character?

The enzyme adenylosuccinate lyase Number of different amino acids types at the given position 234251255325346444563655 In a multiple sequence alignment, does the degree of sequence variation at different positions reflect a potential alpha helical character?

The enzyme adenylosuccinate lyase Number of different amino acids types at the given position 234251255325346444563655 In a multiple sequence alignment, does the degree of sequence variation at different positions reflect a potential alpha helical character?

Porin: a transmembrane beta barrel Residues 213-222: GLN TRP ALA THR GLY LEU LYS TYR ASP ALA

Porin: a transmembrane beta barrel Inside surface: aqueous Outside surface: lipid-facing Residues 213-222: GLN TRP ALA THR GLY LEU LYS TYR ASP ALA