Intracellular Compartments and Vesicular Trafficking

Slides:

Advertisements

Similar presentations

Intracellular Trafficking

Advertisements

Lysosome Nucleus ER Plasma Membrane Mitochondria Golgi.

Chapter 13 Intracellular Vesicular Traffic 張學偉助理教授.

Intracellular Transport1 Chapter 15 You should review functions of different organelles We already discussed evolution; review Focus will be on: 1) mechanisms.

Bulk Transport/Vessicular Traffic Bulk transport refers to the transport of solutes enclosed in vesicles. Many of these solutes are too large to diffuse.

Last Class: 1. transportation at the plasma membrane A. carrier protein, B. channel protein 2. intracellular compartments A. nucleus, B. Endoplasmic Reticulum.

Vesicular transport Dr. med. habil. Kőhidai László Assoc. Professor Depertment of Genetics, Cell- and Immunoibiology Semmelwesi University Semmelwesi University2008.

Protein Sorting ISAT 351, Spring 2004 College of Integrated Science and Technology James Madison University.

Molecular mechanism of vesicular transport Vesicular pathway--Budding and fusion 1.Budding— how are vesicles formed? How can cargo proteins be selectively.

CHAPTER 8 Part 2 Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking Copyright © 2008 by John Wiley & Sons, Inc.

Vesicular Traffic II. Endocytic and secretory pathways red = secretory green = endocytic blue = recycling.

Most proteins in the ER are glycosylated Proteins in cytosol are rarely glycosylated original precursor oligosaccharide added to most proteins in the ER.

Lecture 16 Vesicle transport and targeting in the secretory pathway

Cytoplasmic Membrane Systems II Lecture 12. How Do Proteins Get Imported Into Membrane Enclosed Organelles? Import Requires Input of Energy to Occur!

Cytoplasmic Membrane Systems: Structure, Function, and

Vesicular transport Dr. med. habil. Kőhidai László Assoc. Professor Depertment of Genetics, Cell- and Immunoibiology Semmelwesi University Semmelwesi University.

Lecture 6 - Intracellular compartments and transport I.

Lecture 3 Vesicular Trafficking -Cops and Clathrins -Arfs, Rabs, Sars -Snares.

Protein trafficking between membranes By Graham Warren & Ira Mellman

Extra credit for Midterm 2

Chapter 25 Protein trafficking Introduction 25.2 Oligosaccharides are added to proteins in the ER and Golgi 25.3 The Golgi stacks are polarized.

Previously Bio308 Hypotheses for molecular basis of bipolar disorder Suggest problem lies in protein targeting How are proteins targeted and delivered?

Endocytosis & Exocytosis Pooja Patel Elizabeth Pemberton.

Summary 1.Rough ER and smooth ER; 2.Signal hypothesis, translocation into ER; 3.Single-span and multi-span membrane proteins; 4.Glycosylation; 5.Protein.

The Secretory Pathway Becky Dutch Molecular and Cellular Biochemistry 1. ER - translation 2. ER- protein modifications 3. Discussion Section 4. Golgi apparatus.

Previously Bio308 Hypotheses for molecular basis of bipolar disorder Suggest problem lies in protein targeting How are proteins targeted and delivered?

Endocytosis & Exocytosis By : Matt Sargeant. What is Endocytosis and Exocytosis? Endocytosis is a process by which cells absorb molecules (such as proteins)

Molecular and Cellular Biochemistry 2. ER- protein modifications

Copyright © 2005 Pearson Prentice Hall, Inc. Intracellular Compartments and Transport Membrane Enclosed Organelles Protein Sorting Vesicular Transport.

BIO201A Cell Biology Lecture 29 Wednesday 04/04/07.

Previously Bio308 Hypotheses for molecular basis of bipolar disorder Suggest problem lies in protein targeting How are proteins targeted and delivered?

Previously Bio308 Hypotheses for molecular basis of bipolar disorder Suggest problem lies in protein targeting How are proteins targeted and delivered?

Copyright (c) by W. H. Freeman and Company 17.3 The rough ER is an extensive interconnected series of flattened sacs Figure

Vesicular Transport Pages The general flow of membrane enclosed material within cells.

Vesicular Trafficking Movement From the ER Through the Golgi.

Lecture 12: The secretory pathway

The ER and Golgi Modify & Sort Proteins

1 Chapter 1 Plant Cells Overview  Earth’s primary producers  Nonmotile  Structure to catch light and sense gravity  Structure for transporting water.

How proteins get out of the Golgi apparatus? TGN.

Intracellular Vesicular Traffic

Subcellular compartments that constitute the cellular endomembrane system. The major organelles of the endomembrane system are: the endoplasmic reticulum.

glycosylation is a non-template derived phenomenon

Endomembrane system and vesicular transport

Active Transport Processes

Protein Sorting & Transport

Vesicular Trafficking

Subcellular compartments that constitute the cellular endomembrane system. The major organelles of the endomembrane system are: the endoplasmic reticulum.

Vesicular transport Dr. med. habil. Kőhidai László Assoc. Professor

Intracellular Trafficking

A Closer Look at Cell Membranes

Intracellular Compartments and Transport 2

INTD5000, REVIEW, Lectures C7-C8

Intracellular Transport Pathways

The Mechanisms of Vesicle Budding and Fusion

The Road Taken Cell Volume 100, Issue 1, Pages (January 2000)

COP-coated vesicles Current Biology

Membrane Dynamics in Endocytosis

Membrane Traffic in the Late Steps of Cytokinesis

EXOCYTOSIS, ENDOCYTOSIS & RECEPTOR MEDIATED ENDOCYTOSIS

Coats, Tethers, Rabs, and SNAREs Work Together to Mediate the Intracellular Destination of a Transport Vesicle Huaqing Cai, Karin Reinisch, Susan Ferro-Novick

Ayman El-Sayed, Hideyoshi Harashima Molecular Therapy

Endocytic trafficking of CFTR in health and disease

Tracing the Retrograde Route in Protein Trafficking

Membrane Trafficking in Plant Immunity

A Gate Keeper for Axonal Transport

Chapter 7 Inside the Cell Biological Science, Third Edition

Endocytic and biosynthetic-secretory pathways

Chapter 13 Intracellular Vesicular Traffic.

Neuronal Polarity and Trafficking

Presentation transcript:

Intracellular Compartments and Vesicular Trafficking August 29, 2018 Tom Gallagher tgallag@luc.edu CTRE Room 234 Extension 64850

Four Introductory Slides

Proteins made in the ER are transported in vesicles comprising the exocytic pathway Proteins from the cell exterior are transported in vesicles comprising the endocytic pathway

Vesicle trafficking is continuous, specific, often two-way

Vesicular transport involves: Cargo selection / loading (2) Vesicle budding (3) Vesicle transport (4) Vesicle targeting (5) Vesicle fusion / cargo delivery

Same steps in exocytosis and endocytosis (4) Targeting (5) Fusion exo endo (1) Cargo selection (3) Transport (2) Budding Figure 13-66a Molecular Biology of the Cell (© Garland Science 2008)

Vesicular transport involves: Cargo selection / loading (2) Vesicle budding (3) Vesicle transport (4) Vesicle targeting (5) Vesicle fusion / cargo delivery

Distinct coat proteins are used for secretory and endocytic pathways

COP II vesicles at the transitional ER (anterograde transport)

GTP-binding proteins regulate coat and vesicle assembly Sar1-GAP

COPI vesicles at the Golgi (retrograde transport)

Clathrin vesicles at the trans-Golgi and plasma membranes (endocytosis)

Low Density Lipoprotein (LDL) endocytosis: Clinically important cholesterol regulation

Defective LDL receptors that can not associate in clathrin-coated pits will cause hyper-cholesterolemia

Vesicular transport involves: Cargo selection / loading (2) Vesicle budding (3) Vesicle transport (4) Vesicle targeting (5) Vesicle fusion / cargo delivery

The coat proteins drive most of the vesicle morphogenesis

Dynamin assists with the membrane fission process Alberts, Fifth Ed., Fig. 13-12

Vesicular transport involves: Cargo selection / loading (2) Vesicle budding (3) Vesicle transport (4) Vesicle targeting (5) Vesicle fusion / cargo delivery

The Golgi apparatus is a vesicle transport hub

A central role of the Golgi is to sort and process membrane proteins and secreted proteins

Formation of secretory storage granules

Protein secretion occurs by both a regulated and non-regulated (constitutive) pathway

Vesicular transport involves: Cargo selection / loading (2) Vesicle budding (3) Vesicle transport (4) Vesicle targeting (5) Vesicle fusion / cargo delivery

COPI, COPII and clathrin are removed after vesicle budding: Other factors then control vesicle targeting ?

Phosphotidyl inositols “mark” membrane domains

Rabs and Rab effectors also “mark” membrane domains

Vesicles have diverse “markings” on them

Rabs and Rab effectors direct transport vesicle movement along the cytoskeleton GTP transport vesicle Actin or Microtubule filaments motor protein Rab effector

Rabs and Rab effectors mediate docking and fusion of transport vesicles with their correct target membrane

SNARE proteins contribute to specificity in vesicle targeting and promote vesicle fusion to its target

The SNAREs have to be taken apart to be re-used for another round of membrane fusion

Botulism toxin poisons neurons by cleaving SNAREs; toxin is now re-purposed as “BOTOX”

The steps in vesicular transport Front. Endocrinol., 17 January 2011 | doi: 10.3389/fendo.2011.00001 Rab proteins and the secretory pathway: the case of Rab18 in neuroendocrine cells Rafael Vázquez-Martínez1,2 and Maria M. Malagón1,2*