1. CHAPTER 8 Part 2 Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking Copyright © 2008 by John Wiley & Sons, Inc.

2. ER to the Golgi Complex: The first step in vesicular transport ERGIC Region Visualizing membrane traffic with a fluorescent tag

3. Vesicular Transport from the ER to the Golgi

4. The Golgi Complex

5. Glycosylation in the Golgi Complex No need to memorize the steps; only the concept is important

6. Dynamics of Transport through the Golgi Complex

7. COP1 and COPII Coated Vesicles

8. Vesicular Transport between membranous Compartments of the biosynthetic/secretory pathway

9. Roles of COPII proteins in assembling protein coat and capturing cargo

10. Accumulation of COPI- coated Vesicles

11. Retrieval of Resident ER Proteins !

12. Protein Transport from one Golgi cisternae to another can be assayed in a cell-free system (example: cultured fibroblasts); Fig a & b Fig. a

13. VSV G protein in vitro contains the additional N-acetylglucosamine!!! This modification is carried out by transferase enzyme that is moved by retrograde transport vesicles from the wild-type medial-Golgi cisternae to the mutant cis-Golgi cisternae in the reaction mixture (Science 276:1212, 1997). Fig. b

14. VERY IMPORTANT DIAGRAM ! Read Legend in Text-book; Fig 8.29 Targeting Lysosomal Enzymes To Lysosomes

15. Formation and Fusion of a Transport Vesicle

16. Formation of Calthrin coated Vesicles at the TGN

17. Model for Targeting of Transport Vesicles to Target Membranes

18. Model for interaction between v- and t-SNAREs leading to membrane fusion and exocytosis

19. LYSOSOMES

20. Lysosomes Liver Cells Neuron of a Human with Lysosomal Storage Disorder Accumulation of unidgested glycolipids