1. Vesicular Traffic II

2. Endocytic and secretory pathways red = secretory green = endocytic blue = recycling

3. Different coats are used for different transport steps in the cell

4. Assembly and disassembly of clathrin coat

5. Dynamin pinches clathrin coated vesicles from the membrane

6. Electron micrograph from Drosophila mutant

7. Resting Chemical Synapse

8. Drosophila mutant can not recycle synaptic vesicles

10. SNARE proteins guide vesicular transport

11. Active Chemical Synapse

12. Interaction of SNARE proteins while docking synaptic vesicle at nerve terminal

13. Model for membrane fusion. Tight SNARE pairing forces water molecules from area between lipid bilayers

14. Entry of HIV into lymphocytes

16. Recruitment of cargo molecules into ER transport vesicles

17. Vesicular tubular clusters move along microtubules to carry proteins from ER to Golgi apparatus

18. Vesicular tubular clusters

20. Microtubules

22. DVD Clip 58

23. 3-dimensional model of the Golgi Apparatus

24. Golgi apparatus in an algal cell

25. ER – Golgi transitional zone in an animal cell

26. Some enzymes are enriched in cis or trans compartments of the Golgi substrates specific for certain enzymes such as acid phosphatase

27. Functional Compartmentalization of the Golgi Apparatus

28. Golgi apparatus stained with GFP

29. Golgi apparatus can be polarly distributed In this fibroblast Golgi is facing the direction in which the cell is crawling

30. Drawing of goblet cell in the intestinal epithelium Secretes polysaccharide rich mucus into the small intestine Golgi is highly polarized to facilitate release of mucus through exocytosis at the apical domain

31. N-linked glycosylation because sugar is added to N of asparagine. original precursor oligosaccharide added to most proteins in the ER

32. Oligosaccharide chains are processed in the Golgi common core complex high-mannose

33. Vesicular transport model

34. Cisternal Maturation Model

35. Lysosome interior is different from cytosol

36. Detection of acid phosphatase in lysosomes Small spheres may be vesicles delivering the enzyme from the Golgi Apparatus