1. Most proteins in the ER are glycosylated Proteins in cytosol are rarely glycosylated original precursor oligosaccharide added to most proteins in the ER

2. Protein glycosylation in the rough ER

3. Oligosaccharides are used as tags to mark the state of protein folding

4. Misfolded proteins are exported from ER and destroyed

5. Chapter 13 Intracellular Vesicular Traffic

6. Roadmap of protein traffic inside cell

7. Endocytic and secretory pathways red = secretory green = endocytic blue = recycling

8. 5.14 Figure 5.14 figure 05-14.jpg

9. Vesicular transport

11. There are various types of coated vesicles

12. Different coats are used for different transport steps in the cell

13. Coated Pits are the sites of receptor mediated endocytosis Clathrin protein cytosol Material to be taken up by cell

14. Clathrin-coated pits and vesicles

15. Structure clathrin triskelion

16. Arrangement of triskelion on surface of clathrin coated vesicle

18. DVD Clip 57

19. Assembly and disassembly of clathrin coat

20. Dynamin pinches clathrin coated vesicles from the membrane

21. Electron micrograph from Drosophila mutant

22. Resting Chemical Synapse

23. Drosophila mutant can not recycle synaptic vesicles

25. SNARE proteins guide vesicular transport

26. Active Chemical Synapse

27. Interaction of SNARE proteins while docking synaptic vesicle at nerve terminal

28. Model for membrane fusion. Tight SNARE pairing forces water molecules from area between lipid bilayers

29. Exam I average 81.2% median 84% Scramblase question was not graded Add 1 point to your score