Cloning and expression of rat nesprin-1.

Slides:

Advertisements

Similar presentations

Wu et al., Supplementary Fig 1 Supplementary Figure 1: The depletion of BRG1 in HeLa cells has no effect on cell proliferation. Sh- empty vector was used.

Advertisements

Invest. Ophthalmol. Vis. Sci ;45(6): doi: /iovs Figure Legend:

Rupp et al. Supplementary Figure 1: Structure of the human troponin T gene Exon 6 Genomic DNA cDNA from mRNA mutation Exon 9 Exon bp Parts of genomic.

Effect of miR-29a inhibition on miRNA and SCD expression in GIFT

A B D C E 5 kb 2 kb Copies per 1,000 GAPDH copies. UL US TRL

Novel Cis Element for Tissue-Specific Transcription of Rat Platelet-Derived Growth Factor β-Receptor Gene by Yasunori Takata, Yutaka Kitami, Tomikazu Fukuoka,

IL-1β stimulates CXCL5 and CXCL8 gene expression and protein secretion in A549 cells in a time- and dose-dependent manner. IL-1β stimulates CXCL5 and CXCL8.

SncRNA715 expression in oligodendrocytes.

NRSF regulates expression of endogenous ANP, BNP and α‐skeletal actin in ventricular myocytes. NRSF regulates expression of endogenous ANP, BNP and α‐skeletal.

by Takashi Kasukabe, Junko Okabe-Kado, and Yoshio Honma

Regulation of expression of murine transferrin receptor 2

Fig. 1. Potent and selective down-regulation of KRAS mRNA and protein by AZD4785 in vitro and in vivo. Potent and selective down-regulation of KRAS mRNA.

D3: A Gene Induced During Myeloid Cell Differentiation of Linlo c-Kit+ Sca-1+ Progenitor Cells by Sarah R. Weiler, John M. Gooya, Mariaestela Ortiz, Schickwann.

Volume 3, Issue 4, Pages (October 2014)

Eric J. Stelnicki, Jeff Arbeit, Darrell L

Fig. 1. Schematic representation of the MANO method.

Volume 12, Issue 7, Pages (April 2002)

Erythropoietin gene from a teleost fish, Fugu rubripes

Volume 114, Issue 3, Pages (March 1998)

Volume 94, Issue 6, Pages (September 1998)

Acquisition of a Functional T Cell Receptor during T Lymphocyte Development Is Enforced by HEB and E2A Transcription Factors Mary Elizabeth Jones, Yuan.

Volume 57, Issue 6, Pages (June 2000)

mRNA level as % relative to

Differential expression levels of archaella operon genes and respiratory chain genes in Δsaci_pp2a and Δsaci_ptp. Differential expression levels of archaella.

Quantitative RT-PCR results of relative gene expression normalized to wild-type (WT) expression levels for the LjMYB14 transgene (A) and genes specific.

The human GPR109A promoter is methylated and GPR109A expression is silenced in human colon carcinoma cells. The human GPR109A promoter is methylated and.

Cloning of a novel gene in the human kidney homologous to rat munc13s: Its potential role in diabetic nephropathy Yong Song, Menachem Ailenberg, Mel.

Figure 2 Neuron differentiation and validation

Fig. 5. Effect of gravity and stage on relative expression levels of molecular core clock and clock regulatory genes.Total RNA was isolated from (A) mammary.

Appearance of prominin-1-containing membrane particles in the culture medium upon differentiation of Caco-2 cells. Appearance of prominin-1-containing.

Fig. 4. Effect of gravity and stage on relative expression levels of lipid metabolic and hormone receptor genes.Total RNA was isolated from (A) mammary,

TP53 western blot of primary cultured tail cells from both wild-type (WT) and Tp53Δ11/Δ11 (−/−) rats. TP53 western blot of primary cultured tail cells.

Fig. 1. Lack of Hmga1 and Hmga2 expression in A1/A2-KO mice

Schematic representation of a transcriptomic evaluation approach.

Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse myocardium. Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse.

Fig. 1. IL-31 and IL-31R mRNA expression was upregulated in the asthma mouse model. IL-31 and IL-31R mRNA expression was upregulated in the asthma mouse.

Fig. 3. Coilin levels correlate with altered nascent U2 snRNA, hTR and rRNA levels.RNA isolated from HeLa or WI-38 cells following RNAi targeting coilin.

P53 is responsible for the induction of apoptosis, developmental malformations and mortality of G1 and G2tert mutant zebrafish. p53 is responsible for.

Fig. 6. CBX7 expression in adipocyte differentiation of adipose-derived stem cells.(A) The adipose-derived stem cells, ADS1, were analyzed for the capability.

Elevated expression of vegfaa in hemangiosarcoma.

HSP90β interacts with HNF4A to regulate protein half-life.

Rosiglitazone induces Pdk4 expression in rat and human adipose tissue ex vivo and in 3T3–F442A adipocytes. Rosiglitazone induces Pdk4 expression in rat.

ONC201 activates the ISR. ONC201 activates the ISR. (A) Western blotting analysis for ATF4, CHOP, ATF3, and TRB3 on lysates from HCT116 cells cultured.

Fig. 1. PARN is differentially expressed in breast cancer versus non-cancerous tissue/cells. PARN is differentially expressed in breast cancer versus non-cancerous.

Effect of Dipy on the expression of chondrogenic markers.

Genomic structure of LTBP-4 around the 3rd 8-Cys repeat.

RT-PCR analysis of LTBP expression in human cell lines.

Cdc2 expression in cultured Schwann cells.

Chd1 expression and CHD1 localization during mouse preimplantation development. Chd1 expression and CHD1 localization during mouse preimplantation development.

Microarray analysis of DRG explant cultures of wild-type and ErbB3-deficient mice. Microarray analysis of DRG explant cultures of wild-type and ErbB3-deficient.

KIF13A depletion leads to a drop in virus titers, without affecting viral protein expression. KIF13A depletion leadsto a drop in virus titers, without.

Caveolin-1 downregulated survivin expression in cells with enhanced or constitutive activity via the Wnt pathway. Caveolin-1 downregulated survivin expression.

The influence of SHIP2 on cell migration in breast cancer cells.

Kristiina Airola, Norbert E. Fusenig

The expression pattern of marker proteins for sensory neuron subgroups changes during postnatal maturation. The expression pattern of marker proteins for.

Fig. 1. Potent and selective down-regulation of KRAS mRNA and protein by AZD4785 in vitro and in vivo. Potent and selective down-regulation of KRAS mRNA.

RT-PCR quantification of ubiquitination gene transcripts in rat islets exposed to 2.8 or 16.7 mmol/l glucose for 2 h (A) or 24 h (B). RT-PCR quantification.

Ctip2 directly regulates Notch1 expression in differentiated cells.

APLF regulates genes implicated in MET during the generation of iPSCs from fibroblasts. APLF regulates genes implicated in MET during the generation of.

Fig. 1. DEL-1 is expressed by human and mouse osteoclasts.

RORγt, a Novel Isoform of an Orphan Receptor, Negatively Regulates Fas Ligand Expression and IL-2 Production in T Cells You-Wen He, Michael L Deftos,

TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor tissues. TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor.

Bcl-XL upregulates the expression of genes characteristic to midbrain and hindbrain fates during in vitro differentiation of ES cells. Bcl-XL upregulates.

BORIS mRNA expression in DNMT deficient HCT116 cell lines.

Northern blot analysis of maspin in cancer cell lines and human tissues. Northern blot analysis of maspin in cancer cell lines and human tissues. A, HMECs.

Reduced klotho expression in pancreatic cancer.

Lineage-specific decline of E2F1 nuclear levels during oligodendrocyte differentiation. Lineage-specific decline of E2F1 nuclear levels during oligodendrocyte.

Osteoactivin is overexpressed in aggressively bone metastatic 4T1 subpopulations versus weakly bone metastatic breast cancer populations. Osteoactivin.

Tumorigenicity of GR CAFs

Relative abundance and expression of the 10 most abundant MAGs in the bioreactor at day 96. Relative abundance and expression of the 10 most abundant MAGs.

Presentation transcript:

Cloning and expression of rat nesprin-1. Cloning and expression of rat nesprin-1. (A) Northern blot showing high expression of a 10 kb transcript of rat nesprin-1 (1RA1) in aortic tissue compared with cultured, passage 12, dedifferentiated VSMCs and fibroblasts. (B) Semi-quantitative RT-PCR analysis of rat nesprin-1 (1RA1), showing an increase in expression during developmental differentiation of the aorta. f17, foetal day 17; f19, foetal day 19; n1, neonatal day 1; w2-w8, weeks 2 to 8; ad, adult and c, negative control. The graph shows relative expression of 1RA1 at each stage normalized to a GAPDH control. (C) Schematic representation of isolation of rat nesprin-1 cDNA sequence. Qiuping Zhang et al. J Cell Sci 2001;114:4485-4498 © The Company of Biologists Limited 2001