1. NRSF regulates expression of endogenous ANP, BNP and α‐skeletal actin in ventricular myocytes.
NRSF regulates expression of endogenous ANP, BNP and α‐skeletal actin in ventricular myocytes. (A) Northern blots showing levels of ANP, BNP, α‐skeletal actin (SkA), α‐cardiac actin (CaA) and GAPDH mRNA in cultured ventricular myocytes infected with Ad/lacZ or Ad/dnNRSF for 24 h. (B) A cDNA construct for the generation of dnNRSF Tg mice; Hgh, the poly(A) sequence of human growth hormone gene. (C) Western blot analysis for dnNRSF expression in ventricles from NTg and two different founders lines of dnNRSF Tg mice (Tg471 and 474). (D) Western blot analysis for dnNRSF expression in various organs from dnNRSF Tg mice: H, heart; Lu, lung; L, liver; K, kidney. (E) Representative northern blots showing levels of the indicated mRNAs in dnNRSF Tg and NTg hearts. (F) Bar graph summarizing the relative cardiac levels of the indicated mRNAs (normalized to GAPDH mRNA levels) detected in the northern blots shown in (E); bars represent means ± SEM from three independent experiments; *P < 0.05 versus NTg hearts; n = 6 each. (G) RT–PCR analysis for NRSF and ANP mRNA expression in ventricles from 13.5‐day mouse embryos (ED 13.5) and 20‐week‐old adult mice (AD). (H) Bar graphs showing relative NRSF mRNA levels (normalized to GAPDH mRNA levels) determined by quantitative RT–PCR. Bars represent means ± SEM from three independent assays; *P < 0.05 versus ED13.5.
Koichiro Kuwahara et al. EMBO J. 2003;22:
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