1. Rupp et al.
Supplementary Figure 1: Structure of the human troponin T gene
Exon 6
Genomic DNA
cDNA from mRNA
mutation
Exon 9
Exon 10
302bp
Parts of genomic DNA and cDNA from Troponin T (TnT) are shown. The mutation site (G>A) is located at position 8,728 in exon 9. Primers of human TnT were designed to span from exon 6 to exon 10. The length of the analyzed cDNA is 302bp.

2. Rupp et al.
Supplementary Figure 2: Immunostaining of differentiated CPC
human cell marker
merge
a-sarcomeric
actinin
CM-Dil
a-sarcomeric
actinin
hNA
Immunohistochemistry of co-cultured circulating progenitor cells (CPC) and rat cardiomyocytes after 6 days. The co-culture assay was performed as previously described (7,9). Patient-derived CM-DiL-labelled CPC (upper panel) or human nuclear antigen (hNA) positive cells (lower panel) express α-sarcomeric actinin (green) and display differentiated human cardiomyocytes.

3. Donor A: Sequence of 7 clones
Rupp et al.
Supplementary Figure 3: Wildtyp and mutated sequence of human TnT
Donor A: Sequence of 7 clones #5 #2 #3 #1 #4 #6 #7
Donor B
7 clones derived from RNA of co-cultured CPC of donor A (green box) were sequenced. The wild type human TnT sequence was found in 4 clones. The mutated TnT sequence was found in 3 clones (blue filled box). Sequence of a clone isolated from control (donor B) is shown in red box.

4. Rupp et al.,
Supplementary Figure 4: RT-PCR for cardiospecific markers after co-culture of CPC on fixed cardiomyocytes
h-Nkx2.5
h-MLC-2V
h-ANP
GAPDH CM
Fixed CM
+ CPC
h-TnT
hr-GAPDH
CPC
Fixed CM
+ CPC CM
human
heart
RT-PCR for human TnT in CPC cultivated for 6 days with previously paraformaldehyde- fixed cardiomyocytes (1 %, 15 min). Human TnT is expressed in human heart and in differentiated CPC after co-culture of CPC and cardiomyocytes (CM). Human TnT is not expressed in rat cardiomyocytes and in CPC before co-culture. RT-PCR for additional cardiac marker genes as indicated in CPC after co-culture with paraformaldehyde-fixed rat cardiomyocytes for 6 days is shown in the lower panel. Rat cardiomyocytes served as negative control. Human and rat GADPH was used as loading control.