Fig. 6. RhoB is required for Rnd3-induced stress fibre formation

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Fig. 6. RhoB is required for Rnd3-induced stress fibre formation Fig. 6. RhoB is required for Rnd3-induced stress fibre formation.(A) HUVECs were transfected with siRNAs against RhoA or RhoB either individually or in combination. RhoB is required for Rnd3-induced stress fibre formation.(A) HUVECs were transfected with siRNAs against RhoA or RhoB either individually or in combination. After 48 hours, cells were transfected with plasmid encoding Flag-Rnd3. After 24 hours, cells were fixed and stained with anti-Flag antibody and for F-actin. (A) Representative images of cells. Scale bar: 20 µm. (B,C) Cells were scored for an increase in stress fibre formation compared to untransfected cells. Results for 2 different siRNAs targeting RhoA or RhoB are shown. Western blots showing levels of RhoA and RhoB proteins 72 hours after siRNA transfection are shown below. Data represent the mean ± s.d. of 3 independent experiments; at least 24 cells were counted per experiment. *P≤0.05, **P≤0.01, ***P≤0.001. Undine Gottesbühren et al. Biology Open 2012;bio.20123574 © 2012. Published by The Company of Biologists Ltd