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Fig. 5. Prip silencing enhances the co-localization of GABARAP with insulin vesicles and β-tubulin.Co-localization of GABARAP (green) with insulin (red)

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Presentation on theme: "Fig. 5. Prip silencing enhances the co-localization of GABARAP with insulin vesicles and β-tubulin.Co-localization of GABARAP (green) with insulin (red)"— Presentation transcript:

1 Fig. 5. Prip silencing enhances the co-localization of GABARAP with insulin vesicles and β-tubulin.Co-localization of GABARAP (green) with insulin (red) (A) or β-tubulin (red) (B) in MIN6 cells transfected with scrambled siRNA (upper panels) or Prip siRNA mix [Prip1-siRNAs (1, 2, 3) and Prip2-siRNAs (1, 2, 3), lower panels] after stimulation with 30 mM glucose for 10 min. Prip silencing enhances the co-localization of GABARAP with insulin vesicles and β-tubulin.Co-localization of GABARAP (green) with insulin (red) (A) or β-tubulin (red) (B) in MIN6 cells transfected with scrambled siRNA (upper panels) or Prip siRNA mix [Prip1-siRNAs (1, 2, 3) and Prip2-siRNAs (1, 2, 3), lower panels] after stimulation with 30 mM glucose for 10 min. Magnified images of panels A and B are shown in supplementary material Fig. S9B,C. A set of representative images from 3 independent experiments is shown. The overlap coefficient was calculated. Values are presented as mean ± s.d. (from left in each graph of panels A and B; n = 144 and 112; 84 and 160, respectively); ***p< Scale bars: 5µm. Satoshi Asano et al. Biology Open 2014;bio © Published by The Company of Biologists Ltd

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