1. Fig. 4. Expression of p75NTR and time-course of TrkA autophosphorylation at the Y751 and Y490 sites in PC12-27 cells transfected with the vector, empty (PC12-27/Ctrl) or including the full length p75NTR (PC12-27/p75NTR).(A) The western blot of p75NTR in wtPC12, PC12-27/Ctrl and PC12-27/p75NTR cells.
Expression of p75NTR and time-course of TrkA autophosphorylation at the Y751 and Y490 sites in PC12-27 cells transfected with the vector, empty (PC12-27/Ctrl) or including the full length p75NTR (PC12-27/p75NTR).(A) The western blot of p75NTR in wtPC12, PC12-27/Ctrl and PC12-27/p75NTR cells. Quantization of the data, documenting the similar levels of the receptor in the wtPC12 and PC12-27/p75NTR cells, is on the right. (B) The surface immunolocalization of p75NTR in the PC12-27/Ctrl and PC12-27p75NTR cells. The quantization of the results is on the right. Scale bar: 10 µm. (C,D) The time-course of the TrkA autophosphorylation at the Y751 and Y490 sites induced by NGF (100 ng/ml) in the two transfected subclones, PC12-27/Ctrl and PC12-27/p75NTR. The quantization of these data is shown on the right. Statistical analysis and significance of the differences is given as specified in the legend for Fig. 1.
Sara Negrini et al. Biology Open 2013;2:
© Published by The Company of Biologists Ltd