αS1-Casein elucidate major T-cell responses in cow's milk allergy

Slides:

Advertisements

Similar presentations

Early exposure to cow's milk protein is protective against IgE-mediated cow's milk protein allergy Yitzhak Katz, MD, Nelly Rajuan, MSc, Michael R. Goldberg,

Advertisements

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy Erik Wambre, PhD, Jonathan.

Specific immunotherapy modifies allergen-specific CD4+ T-cell responses in an epitope- dependent manner Erik Wambre, PhD, Jonathan H. DeLong, BSc, Eddie.

Comparison of the allergic and nonallergic CD4+ T-cell responses to the major dog allergen Can f 1 Suvi Parviainen, MSc, Antti Taivainen, MD, PhD, Aino.

Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.

Joseph A. Odhiambo, MMed, Hywel C. Williams, PhD, Tadd O

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion

Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu Amanda J. Reefer, MS, Kathryn.

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

Ann-Marie M. Schoos, MD, PhD, Jacob D

Allergy prevention starts before conception: Maternofetal transfer of tolerance protects against the development of asthma Tobias Polte, PhD, Christian.

CD4+ T cells in patients with chronic inflammatory rheumatic disorders show distinct levels of exhaustion Theresa Frenz, PhD, Elena Grabski, PhD, Daniela.

Immunologic defects in 22q11.2 deletion syndrome

Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy Julie Wang, MD,

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion

Identification of a subset of human natural killer cells expressing high levels of programmed death 1: A phenotypic and functional characterization Silvia.

Type 3 innate lymphoid cells induce proliferation of CD94+ natural killer cells Shuo Li, PhD, Hideaki Morita, MD, PhD, Beate Rückert, Sci Tec, Tadech.

Is 9 more than 2 also in allergic airway inflammation?

Children with allergic and nonallergic rhinitis have a similar risk of asthma Bo Lund Krogsgaard Chawes, MD, Klaus Bønnelykke, MD, PhD, Eskil Kreiner-Møller,

In vivo allergenic activity of a hypoallergenic mutant of the major fish allergen Cyp c 1 evaluated by means of skin testing Nikolaos Douladiris, MD,

Characterization of human memory CD4+ T-cell responses to the dog allergen Can f 4 Aino L. Rönkä, MD, Tuure T. Kinnunen, MD, PhD, Amélie Goudet, BSc,

Lieuwe D. Bos, MSc, PhD, Peter J. Sterk, MD, PhD, Stephen J

Jug r 2–reactive CD4+ T cells have a dominant immune role in walnut allergy Luis Diego Archila, MSc, David Jeong, MD, Mariona Pascal, PhD, Joan Bartra,

Kathleen R. Bartemes, BA, Gail M. Kephart, BS, Stephanie J

Katherine G. MacDonald, BSc, Nicholas A. J

Thymic stromal lymphopoietin converts human epidermal Langerhans cells into antigen- presenting cells that induce proallergic T cells Susanne Ebner, PhD,

Elevated regulatory T cells at diagnosis of Coccidioides infection associates with chronicity in pediatric patients Dan Davini, BA, Fouzia Naeem, MD,

Peter M. Wolfgram, MD, David B. Allen, MD

Joseph A. Odhiambo, MMed, Hywel C. Williams, PhD, Tadd O

Greater epitope recognition of shrimp allergens by children than by adults suggests that shrimp sensitization decreases with age Rosalía Ayuso, MD, PhD,

Patients with atopic dermatitis and history of eczema herpeticum elicit herpes simplex virus–specific type 2 immune responses Stephan Traidl, Petra Kienlin,

Allison K. Martin, BS, Douglas G. Mack, PhD, Michael T

Volume 127, Issue 3, Pages (September 2004)

A thymic stromal lymphopoietin–responsive dendritic cell subset mediates allergic responses in the upper airway mucosa Guro R. Melum, MD, Lorant Farkas,

Benjamin T. Prince, MD, Msci, Ashley L. Devonshire, MD, MPH, Kristin A

Specific immunotherapy modifies allergen-specific CD4+ T-cell responses in an epitope- dependent manner Erik Wambre, PhD, Jonathan H. DeLong, BSc, Eddie.

Chronic cat allergen exposure induces a TH2 cell–dependent IgG4 response related to low sensitization Amedee Renand, PhD, Luis D. Archila, MSc, John.

Brent E. Palmer, PhD, Douglas G. Mack, PhD, Allison K

β-Agonist enhances type 2 T-cell survival and accumulation

Reply Journal of Allergy and Clinical Immunology

Zoulfia Allakhverdi, PhD, Michael R. Comeau, BSc, Dirk E

Grass tablet sublingual immunotherapy downregulates the TH2 cytokine response followed by regulatory T-cell generation Abel Suárez-Fueyo, PhD, Tania.

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy Erik Wambre, PhD, Jonathan.

Antigen presentation of the immunodominant T-cell epitope of the major mugwort pollen allergen, Art v 1, is associated with the expression of HLA-DRB1∗01

Staphylococcal enterotoxin A–activated regulatory T cells promote allergen-specific TH2 response to intratracheal allergen inoculation Wei-ping Zeng,

Direct ex vivo analysis of allergen-specific CD4+ T cells

Autophagy: Nobel Prize 2016 and allergy and asthma research

Tuure Kinnunen, MD, Kalle Jutila, BM, William W

Influence of early-life exposures on food sensitization and food allergy in an inner-city birth cohort Emily C. McGowan, MD, Gordon R. Bloomberg, MD,

Allergen-specific CD8+ T cells in peanut-allergic individuals

Sphingosine-1-phosphate/sphingosine-1-phosphate receptor 1 signaling is required for migration of naive human T cells from the thymus to the periphery

Association between specific timothy grass antigens and changes in TH1- and TH2-cell responses following specific immunotherapy Véronique Schulten, PhD,

CD23 surface density on B cells is associated with IgE levels and determines IgE- facilitated allergen uptake, as well as activation of allergen-specific.

Sara Paveglio, PhD, MS, Erin Bennett, MS, Kelly L. Hawley, PhD, Adam P

Allergen-specific immunotherapy modulates the balance of circulating Tfh and Tfr cells Véronique Schulten, PhD, Victoria Tripple, BSc, Grégory Seumois,

Defining antigen-specific responses with human MHC class II tetramers

Chemical constituents of diesel exhaust particles induce IL-4 production and histamine release by human basophils Gilles Devouassoux, MDa, Andrew Saxon,

Wasp venom immunotherapy expands a subpopulation of CD4+CD25+ forkhead box protein 3–positive regulatory T cells expressing the T-cell receptor Vβ2 and.

CCR4 and CCR5 expression in conjunctival specimens as differential markers of TH1/ TH2 in ocular surface disorders Christophe Baudouin, MD, PhD, Hong.

Atopic endotype in childhood

Eric B. Brandt, PhD, Melissa K. Mingler, MS, Michelle D

Macrolide antibiotics and asthma treatment

Innate immune defects correlate with failure of antibody responses to H1N1/09 vaccine in HIV-infected patients Suresh Pallikkuth, PhD, Sudheesh Pilakka.

Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.

Gastrointestinal digestion of Bet v 1–homologous food allergens destroys their mediator- releasing, but not T cell–activating, capacity Eva Maria Schimek,

Natural history of cow’s milk allergy

Eosinophil progenitor levels are increased in patients with active pediatric eosinophilic esophagitis David W. Morris, MD, Emily M. Stucke, BA, Lisa.

Invariant natural killer T cells from children with versus without food allergy exhibit differential responsiveness to milk-derived sphingomyelin Soma.

Comparison of the allergic and nonallergic CD4+ T-cell responses to the major dog allergen Can f 1 Suvi Parviainen, MSc, Antti Taivainen, MD, PhD, Aino.

Der p 1–induced CD4+FOXP3+GATA3+ T cells have suppressive properties and contribute to the polarization of the TH2-associated response Lieke Reubsaet,

Presentation transcript:

αS1-Casein elucidate major T-cell responses in cow's milk allergy L. Diego Archila, PhD, Fatima S. Khan, MD, Nupur Bhatnagar, PhD, David Robinson, MD, Mary L. Farrington, MD, William W. Kwok, PhD Journal of Allergy and Clinical Immunology Volume 140, Issue 3, Pages 854-857.e6 (September 2017) DOI: 10.1016/j.jaci.2017.02.028 Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Frequency and phenotype of Bos d-epitope–specific CD4+ T cells. A, Frequencies of Bos d-epitope–specific CD45RA−CD4+ T cells in subjects with and without CMA. B, Comparison of Bos d–specific CD4+ T-cell frequency for each epitope described (data point) between milk-allergic (n = 23; filled squares) and nonallergic subjects (n = 13; opened circles) and adults, teenagers, and children older than 8 years (n = 16; triangles) and children 8 years or younger (n = 7; downside triangles) with CMA. C, PBMCs from a nonallergic and allergic subjects were stained with phycoerythrin-labeled DRB1*10:01/Bos d 9145-164 tetramers and a panel of antibodies. The percentages of memory Bos d–specific T cells that expressed the surface marker of interest are as indicated. D, Phenotype of Bos d–specific T cells in allergic and nonallergic subjects and in (E) children with CMA older than 8 years and children with CMA 8 years or younger. Symbols in Fig 1, D and E, are identical to those used in Fig 1, B. A t test was used in the statistical analysis. NS, Not significant. *P < .05, **P < .01, and ***P < .001. Journal of Allergy and Clinical Immunology 2017 140, 854-857.e6DOI: (10.1016/j.jaci.2017.02.028) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Cytokine profiles of Bos d–reactive T cells. A, First row, Cytokine profile in a DRB1*01:01 nonallergic subject. Second row, Cytokine profile in a DRB1*01:01 allergic adult. Third row, Cytokine profile in a DRB1*01:01 allergic child. The percentages of memory CD154+ Bos d–reactive cytokine-producing T cells are as indicated. B and C, Cytokine profiles of Bos d–reactive T cells in nonallergic (n = 13) and allergic subjects (n = 21), and between adults, teenagers, and children older than 8 (n = 14) and children 8 years or younger (n = 7) with CMA. Data are presented as the mean frequency of cytokine-producing T cells from each group in pie charts. Journal of Allergy and Clinical Immunology 2017 140, 854-857.e6DOI: (10.1016/j.jaci.2017.02.028) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Tetramer-guided epitope-mapping studies of DRB1*10:01-restricted Bos d–specific CD4+ T cells. A, PBMCs from a DRB1*10:01 subject with milk allergy were stimulated with 5 pools of Bos d 11 peptides for 2 weeks and subsequently stained with corresponding DRB1*10:01/Bos d 11 pooled peptide tetramers. B, Cells that were stimulated with pool no. 1 were restained with individual peptides from the corresponding pool. The staining identified p4 (Bos d 1125-44) as DRB1*10:01-restricted Bos d 11 T-cell epitopes. Journal of Allergy and Clinical Immunology 2017 140, 854-857.e6DOI: (10.1016/j.jaci.2017.02.028) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 Frequencies of milk allergen reactive CD4+ T cells. A and B, Frequencies of β-lactoglobulin-, αS1-casein-, αS2-casein-, β-casein-, and κ-casein-reactive T cells in 8 subjects with milk allergy (adults, teenagers, and children >8 years of age n = 6; children ≤8 years of age n = 2) with CD154 assays after 3-hour Bos d peptide stimulation using freshly isolated PBMCs. Each data point represents the frequency of T cells reactive to each allergen. An ANOVA test (with Bonferroni correction) was used to compare all columns in the statistical analysis. NS, Not significant. *P < .05, **P < .01, and ***P < .001. Journal of Allergy and Clinical Immunology 2017 140, 854-857.e6DOI: (10.1016/j.jaci.2017.02.028) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 Phenotypes of CCR6+Bos d–specific T cells in milk-allergic subjects after ex vivo pMHC-II tetramer enrichment using freshly isolated PBMCs. A and B, Tetramer+CD45RA− T cells were gated against CCR4 and CCR6. Each data point represents results for surface expression in tetramer-positive T cells from 23 subjects with milk allergy: 15 adults, teenagers, and children older than 8 years (triangles) and 8 children 8 years or younger (downside triangles) with CMA. C and D, Tetramer-positive CD45RA− T cells were gated against CCR6 and CRTH2. Each data point represents results for surface expression in tetramer-positive T cells from 26 subjects with milk allergy: 18 adults, teenagers, and children older than 8 years (triangles) and 8 children 8 years or younger (downside triangles) with CMA. A t test was used in the statistical analysis. NS, Not significant. *P < .05, **P < .01, and ****P < .0001. Journal of Allergy and Clinical Immunology 2017 140, 854-857.e6DOI: (10.1016/j.jaci.2017.02.028) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions