Two-photon imaging of GCaMP6s in zebrafish larvae.

Slides:

Advertisements

Similar presentations

Mitochondria localize to both the front and the rear of neutrophils.

Advertisements

Alessandra Maria Casano, Marvin Albert, Francesca Peri Cell Reports

Microglia Colonization of Developing Zebrafish Midbrain Is Promoted by Apoptotic Neuron and Lysophosphatidylcholine Jin Xu, Tienan Wang, Yi Wu, Wan Jin,

Direct fusion lines allow fluorescent visualization of plasmatocyte nuclei, the cytoplasm, or the cytoskeleton in embryos from stage (St) 8 onwards. Direct.

Real-Time Visualization of Neuronal Activity during Perception

Incidence and spatial extent of epileptiform events observed in widefield calcium imaging. Incidence and spatial extent of epileptiform events observed.

Distribution and functionality of Arch-EGFP pumps in the peripheral sensory pathways of Nav1.8-Arch+ mice. Distribution and functionality of Arch-EGFP.

Alessandra Maria Casano, Marvin Albert, Francesca Peri Cell Reports

Parametric Functional Maps of Visual Inputs to the Tectum

Fiber innervation is reduced in both central and peripheral targets of DRG neurons in Nav-Tsc2 mice. Fiber innervation is reduced in both central and peripheral.

Brain-wide network synchronization.

ABLE detects synchronously spiking, densely packed cells from mouse in vitro imaging data. ABLE detects synchronously spiking, densely packed cells from.

Examining effects of different microscopes to image clear brains.

The Cellular Organization of Zebrafish Visuomotor Circuits

Light illumination excites Lamina I SDH neurons after PNI

GlyR activation modulates its diffusion properties around active synapses. GlyR activation modulates its diffusion properties around active synapses. A,

The effect of ETC duration and temperature on imaging depth.

Volume 81, Issue 6, Pages (March 2014)

Volume 27, Issue 12, Pages e5 (June 2017)

HDAC4N increases with Braak stage in CA2 hippocampal pyramidal cells.

Volume 24, Issue 4, Pages (February 2014)

Altered development of small-worldness in pcdh19 mutants.

Development of basic network properties in wild-type and pcdh19 mutant larvae. Development of basic network properties in wild-type and pcdh19 mutant larvae.

Microglia-synapse interactions in mice following LPS injections.

p-S6 S240/244 is observed across the V-SVZ lineage.

Developmental changes in transitivity in pcdh19 mutants.

Developmental time course of network measures.

Expression of pcdh19 in BAC transgenic line.

Development of assortativity in wild-type and pcdh19 mutant larvae.

Uncaging responses in spines that are below the optical measurement threshold: clear optical bAP measurements serve as positive controls and for signal.

Stress does not affect the volume fraction of fine astrocytic protrusions across the BA and the dCA3 area of the hippocampus. Stress does not affect the.

Protein expression of EAAT2 paralogs.

PTX-induced reporter protein expression in the brain of the IEG reporter line. PTX-induced reporter protein expression in the brain of the IEG reporter.

Global cell proliferation replenishes epidermal cells.

CIS causes a reduction in GFAP-positive astrocytes in BA but not in dCA3. CIS causes a reduction in GFAP-positive astrocytes in BA but not in dCA3. A,

IANX transiently increased cortical responses to maxillary molar pulp stimulation. IANX transiently increased cortical responses to maxillary molar pulp.

Activation of glial cells.

Effect of FGF8 on R-C marker expression.

GCaMPs in Drosophila. GCaMPs in Drosophila. A, Schematic of larval NMJ preparation, and close-up of Type 1b boutons from muscle 13 (segments A3–A5) used.

RA signaling in early postnatal PFC

Temporal origins of h-ChCs labeled in Cdh6-CreER;Dlx5/6-Flp;Ai65 mice.

c-Fos in RFMes GABAergic neurons across groups.

Neuronal and glial expression of Nogo-A and synaptic localization of Nogo-A and NgR 1 in the motor cortex. Neuronal and glial expression of Nogo-A and.

Purkinje cells inhibit large fastigial glycinergic neurons

Top row, Example high-resolution deconvolved optical z-stack of a dendritic segment used for spine analysis with NeuronStudio software. Top row, Example.

TeNT did not affect lysosome fusion.

3DMorph workflow. 3DMorph workflow. The user selects either Interactive or Automatic mode. Interactive mode must be used first to generate a parameters.

IANX transiently decreased the cortical responses to the mandibular molar pulp stimulation. IANX transiently decreased the cortical responses to the mandibular.

Identification of the mPOA as a downstream target of AGRP neurons.

Nmb cells in rostral parafacial region and dorsal pons are not catecholaminergic. Nmb cells in rostral parafacial region and dorsal pons are not catecholaminergic.

PreBötC activity in low [K+]o implicates neuronal activity from both subpopulations. PreBötC activity in low [K+]o implicates neuronal activity from both.

Ribosomal RNA and β-catenin mRNA localize to newly formed presynaptic terminals. Ribosomal RNA and β-catenin mRNA localize to newly formed presynaptic.

Chronic stress causes a reduction in the neuropil volume occupied by astrocytes in BA but not in dCA3. Chronic stress causes a reduction in the neuropil.

Knock-out of NSG2 decreases mEPSC frequency.

Optogenetic analysis of mPFC neurons.

At 1 month, control and les rats were fed AL or fasted every other day for IF. Representative images of 1 μm sections, with electron micrograph insets.

3D visualization of fluoro-Ruby-labeled CST axons in cleared nonhuman primate spinal cord. 3D visualization of fluoro-Ruby-labeled CST axons in cleared.

SSC LTP in narrow-field vertical neurons is induced by SO stimulation at 20 Hz for 20 s. sSC LTP in narrow-field vertical neurons is induced by SO stimulation.

Seizure propagation across the whole-brain.

Effects of the genotypes of Rapgef2 and Rapgef6 on the apical surface structures. Effects of the genotypes of Rapgef2 and Rapgef6 on the apical surface.

Thy1S promoter drives sparse expression of hrGFP in marmoset cortex.

A tph2 promoter fragment labels DR serotonergic neurons.

MSCaTs measured by GCaMP6f imaging reflect NMDAR activation at individual synapses following spontaneous single vesicle release. mSCaTs measured by GCaMP6f.

Volume 25, Issue 6, Pages (March 2015)

Spatial distribution of node-based measures in a 6 dpf larva.

Passive immunization with CT α-syn antibodies improved TH pathology and neuroinflammation in the striatum of α-syn tg mice. Passive immunization with CT.

Expression of Rapgef2 and Rapgef6 in the E13.5 and E15.5 brains.

Perineurial glia respond to rostral motor nerve injury.

Molecular Spies for Bioimaging—Fluorescent Protein-Based Probes

Activation and expression of the transgene.

Presentation transcript:

Two-photon imaging of GCaMP6s in zebrafish larvae. Two-photon imaging of GCaMP6s in zebrafish larvae. A, Each panel shows an optical section from a single time point of a movie collected in a 4 dpf zebrafish larva. Sections are arranged from dorsal (0 μm) to ventral (–110 μm). The brain was cropped to remove skin fluorescence and signal from the synaptic neuropil of the optic tectum. Scale bar = 100 μm. B, Detection of cells identified by constrained non-negative matrix factorization (Pnevmatikakis et al., 2016). ROIs outlined in magenta on top of an average image for a single imaging plane from a 4 dpf larva. Cb, cerebelleum; Hb, hindbrain; OT, optic tectum. Scale bar = 100 μm. C, Selected ROIs. Scale bar = 15 μm. D, Selected ΔF/F traces obtained from the segmented plane in B, C. Sarah E.W. Light, and James D. Jontes eNeuro 2019;6:ENEURO.0420-18.2019 ©2019 by Society for Neuroscience