1. GlyR activation modulates its diffusion properties around active synapses.
GlyR activation modulates its diffusion properties around active synapses. A, Representative images of QD-labeled endogenous GlyRs (magenta) and FM4-64-labeled active synapses (green) shown as maximum intensity projections of 30 s of image recordings in Ctrl, cSTR, and WASH conditions (top panels). The bottom panels show the trajectories of correspondent QD-labeled GlyRs (centroids) shown in the top panels inside (green) and outside (magenta) active synapses. Scale bars, 2 µm. B, Cumulative probabilities of the median diffusion coefficient of each QD-GlyR within the active synapse region in Ctrl (n = 101 particles, 7 cells), cSTR (n = 125 particles, 8 cells), and WASH (n = 54 particles, 7 cells) conditions. C, Median diffusion coefficients of each QD-GlyR at synapses comparing Ctrl, cSTR, and WASH conditions (median ± 25–75% interquartile range (IQR)). D, Average dwell times of QD-GlyRs within synapse regions (Ctrl: n = 150 particles, 7 cells; cSTR: n = 314 particles, 8 cells; WASH: n = 97 particles, 7 cells, mean ± SEM). E, Average confinement size of QD-GlyRs at synapses (mean ± SEM) in Ctrl (n = 49 particles, 7cells), cSTR (n = 38 particles, 8 cells), and WASH (n = 19 particles, 7 cells) conditions. Significance of difference was tested by Kruskal–Wallis test followed by post hoc comparisons using the Mann–Whitney U test with Bonferroni’s correction, ***p < 0.001/3 after Bonferroni’s correction for the three tests (C) and by one-way ANOVA with Bonferroni post hoc test (D, E). **p < 0.01, ***p < n.s., Not significant.
Yoshihisa Nakahata et al. eNeuro 2017;4:ENEURO
©2017 by Society for Neuroscience