1. Examining effects of different microscopes to image clear brains.
Examining effects of different microscopes to image clear brains. Although a light sheet or confocal microscope may be ideal, when sufficiently clear, tissue may be imaged using even a very basic epifluorescent microscope. In this case, a Nikon Eclipse 80i with a mercury arc lamp was used to image a 2-mm-thick cleared section. A–F, Single image planes at 100 µm intervals through the z-stack. Bright and sharp cells can be seen through at least 600 µm of the tissue.
Jonathan R. Epp et al. eneuro 2015;2:ENEURO
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