CD27 expression on bovine γδ and CD4 T cells ex vivo and in response to mitogen. CD27 expression on bovine γδ and CD4 T cells ex vivo and in response to.

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CD27 expression on bovine γδ and CD4 T cells ex vivo and in response to mitogen. CD27 expression on bovine γδ and CD4 T cells ex vivo and in response to mitogen. Using flow cytometry, bovine PBMCs from healthy cows were analyzed for CD4 or γδ TCR expression. Gating hierarchy (gating sequence as depicted by the arrows): live cells (gate 1), lymphocytes (gate 2), and γδ or CD4 T cells (gate 3). Each lymphocyte population was further analyzed for CD27 expression following mock or PWM stimulation for 6 d (dot plots). Data are representative of n = 12 animals from six independent experiments. FMO, fluorescence minus one; FSC-A, forward scatter A; SSC-A, side scatter A; SSC-H, side scatter H. Mariana Guerra-Maupome et al. ImmunoHorizons 2019;3:208-218 Copyright © 2019 The Authors