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Flow cytometry analysis of TNF-β- and IL-10-producing CD33+ cells.

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Presentation on theme: "Flow cytometry analysis of TNF-β- and IL-10-producing CD33+ cells."— Presentation transcript:

1 Flow cytometry analysis of TNF-β- and IL-10-producing CD33+ cells.
Flow cytometry analysis of TNF-β- and IL-10-producing CD33+ cells. PBMCs cultured for 48 h in medium (left set of panels under “control”) or stimulated with PMA-ionomycin (middle set of panels) or PHA (right set of panels) in the presence of monensin were labeled for surface CD33 (PE/Cy5) and intracellular TNF-β or IL-10. Cells were gated according to CD33 expression and SSC (region R1). Histogram overlays show FL2 (orange fluorescence) intensity corresponding to a given cytokine (solid line) compared to the intensity for the isotype-specific control (dotted line). Results from one representative experiment of five experiments performed are shown. Jarołsaw Baran et al. Clin. Diagn. Lab. Immunol. 2001; doi: /CDLI

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