1. Depletion of CD169+ cells leads to increased expression of CD25 on enterotoxin A–specific Vβ3+ T cells.
Depletion of CD169+ cells leads to increased expression of CD25 on enterotoxin A–specific Vβ3+ T cells. WT and CD169-DTR mice received DT 2 d prior to enterotoxin A or vehicle inhalation. Lung and spleen were removed 4 h after vehicle (0 μg) or enterotoxin A (0.033, 0.1, 0.33 or 1 μg) inhalation. (A and B) Representative flow cytometry plots (A) and scatter dot plot (B) show depletion of AMs in lung (gated as live CD45+CD11c+Siglec F+CD169+ cells) in CD169-DTR mice compared with WT mice. The percentage of AMs is relative to all live cells. (C) Representative histogram of CD69 expression in live CD45+CD3+Vβ3+ cells in WT (black) and CD169-DTR (red) mice 4 h after μg of enterotoxin A inhalation. Gray, isotype control. (D) Percentage of CD69+ cells in Vβ3+ (left) and Vβ14+ (right) T cells 4 h after 0, 0.033, 0.1, 0.33, or 1 μg of enterotoxin A inhalation. (E) Representative histogram of CD25 expression in live CD45+CD3+Vβ3+ cells in WT (black) and CD169-DTR (red) mice 4 h after μg of enterotoxin A inhalation. Gray, isotype control. (F) Percentage of CD25+ cells in Vβ3+ (left) and Vβ14+ (right) T cells 4 h after 0, 0.033, 0.1, 0.33, or 1 μg of enterotoxin A inhalation. The data were compiled from three independent experiments with a total of n = 5–6 per group. A two-way ANOVA with a Sidak test was used: *p < 0.05, **p < 0.01, ***p <
Julia Svedova et al. ImmunoHorizons 2017;1:
Copyright © 2017 The Authors