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Figure S2 Gating strategy of AT2 cells based on EpCAM and MHCII

Published byThibaud Soucy Modified over 5 years ago

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Presentation on theme: "Figure S2 Gating strategy of AT2 cells based on EpCAM and MHCII"— Presentation transcript:

1 Figure S2 Gating strategy of AT2 cells based on EpCAM and MHCII
84.8 FSC SSC Live cells 86.6 7-AAD FSC singlet 95.5 FSC-Hight FSC-Width SSC singlet 92.0 SSC-Height SSC-Width CD45-CD31- cells 28.1 CD45,CD31-PE-Cy7 RatIgG2bκ,RatIgG2aκ-PE-Cy7 RatIgG2aκ-APC RatIgG2bκ-eFluor450 EpCAM-APC P1 89.3 P2 1.6 P3 2.6 MHCII-eFluor450 Figure S2 Gating strategy for isolation of AT2 cells based on EpCAM and MHCII expression Gating strategy for isolation of AT2 cells from single cell suspension of the lungs is demonstrated. Gating of each cell population was performed along the order of arrows. Dotted arrows indicate plots with relevant isotype controls.

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A. a). d). c). f). b).e). g). Figure S1. NAT2 expression in lymphocytes and monocytes from a healthy volunteer. PBMC from a healthy volunteer were simple.

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