Presentation is loading. Please wait.

Presentation is loading. Please wait.

A B Supplemental Figure 1 PBMC Cervical Cytobrush

Published byPercival Grant Modified over 5 years ago

Similar presentations

Presentation on theme: "A B Supplemental Figure 1 PBMC Cervical Cytobrush"— Presentation transcript:

1 A B Supplemental Figure 1 PBMC Cervical Cytobrush
CD CD8+ (CD3+/CD4-) PBMC Cervical Cytobrush B Participant Participant 19 UV-mock UV-HSV-2 Supplemental Figure 1 A. Gating strategy to phenotype total T cells derived from a cervical cytobrush and PBMC sample from a representative HSV-2 infected participant. Gating focused on live CD3+ cells in the lymphocyte forward/side scatter regions that were CD4+ and CD4-negative, the latter of which was considered CD8+ for quantitation. The expression of CCR7/CD45RA and CD69/CD103 are displayed for the CD3+CD4+ and CD3+CD4- (CD8+) populations. B. Measuring cervical cytobrush-derived HSV-2 reactive CD4+ T cell responses from 2 HSVneg participants. Cells isolated from cervical cytobrushes obtained from 2 HSVneg participants were cultured with UV-mock (bottom graphs) or UV-HSV-2 (top graphs) and the resultant CD4+ T cells were analyzed by ICS and flow cytometry for the expression of IFN-g. Net % CD4+/IFNg+ (UV-HSV-2 minus UV-mock) was 0.0% for Participant 18 and 0.01% for Participant 19. In both cases, the proportion of CD4+/IFN-g+ cells stimulated with UV-HSV-2 was not statistically significantly different than those stimulated with UV-mock (Fisher’s Exact Test P>0.05).

Download ppt "A B Supplemental Figure 1 PBMC Cervical Cytobrush"

Similar presentations

Early HIV cohort: Frozen PBMC samples from all 37 HIV-1 infected subjects; (77% of subjects within 6 months of seroconversion) (HIV negative or control.

Early HIV cohort: Frozen PBMC samples from all 37 HIV-1 infected subjects; (77% of subjects within 6 months of seroconversion) (HIV negative or control.
Supplemental Figure 1 A. EpCAM Gating Strategy Top Row: Histogram showing unstained BT474 cells with low fluorescent intensity of the EpCAM specific PE.

Supplemental Figure 1 A. EpCAM Gating Strategy Top Row: Histogram showing unstained BT474 cells with low fluorescent intensity of the EpCAM specific PE.
Supplemental Fig. 1. Gating strategy to define Treg and iNKT cell subsets. (A) Representative scatter plots showing: the lymphocyte gate (R1), CD4+ cells.

Supplemental Fig. 1. Gating strategy to define Treg and iNKT cell subsets. (A) Representative scatter plots showing: the lymphocyte gate (R1), CD4+ cells.
IFNγIL-2TNFaIL-17A CD40 L MOPP DMSO CD4+ Supplemental figure 5A SEB isotype control isotype control isotype control CD69+ A.

IFNγIL-2TNFaIL-17A CD40 L MOPP DMSO CD4+ Supplemental figure 5A SEB isotype control isotype control isotype control CD69+ A.
Suppl. Fig x106 Culture of Irradiated A-CFSE

Suppl. Fig x106 Culture of Irradiated A-CFSE
Vitamin D3 treatment of vitamin D–insufficient asthmatic patients does not alter immune cell function  Brandy Reid, MS, Pierre-Olivier Girodet, MD, PhD,

Vitamin D3 treatment of vitamin D–insufficient asthmatic patients does not alter immune cell function  Brandy Reid, MS, Pierre-Olivier Girodet, MD, PhD,
Fig. 2. Comparison of the Th1/Th2 ratio and the percentages of Th1 and Th2 cells between normal and allergic individuals. (A) The Th1/Th2 ratio observed.

Fig. 2. Comparison of the Th1/Th2 ratio and the percentages of Th1 and Th2 cells between normal and allergic individuals. (A) The Th1/Th2 ratio observed.
MicroRNA-21 Negatively Regulates Treg Cells Through a TGF-β1/Smad-Independent Pathway in Patients with Coronary Heart Disease Cell Physiol Biochem 2015;37:866-878.

MicroRNA-21 Negatively Regulates Treg Cells Through a TGF-β1/Smad-Independent Pathway in Patients with Coronary Heart Disease Cell Physiol Biochem 2015;37:
Figure 1. Phenotype of CD8+ T cells and CD4+ T cells in human immunodeficiency virus (HIV)–seropositive women who are seropositive.

Figure 1. Phenotype of CD8+ T cells and CD4+ T cells in human immunodeficiency virus (HIV)–seropositive women who are seropositive.
Figure 1. Overexpression of PD-1 decoy increases IFN-γ secretion from T cells. Activated B6 splenic T cells were transduced with retrovirus carrying either.

Figure 1. Overexpression of PD-1 decoy increases IFN-γ secretion from T cells. Activated B6 splenic T cells were transduced with retrovirus carrying either.
A All patients Arm A Arm B Deaths/patients 21/30 7/10 14/20

A All patients Arm A Arm B Deaths/patients 21/30 7/10 14/20
Figure e-1A.

Figure e-1A.
The Effect of Antiretroviral Naïve HIV-1 Infection on the Ability of Natural Killer Cells to Produce IFN-γ upon Exposure to Plasmodium falciparum-Infected.

The Effect of Antiretroviral Naïve HIV-1 Infection on the Ability of Natural Killer Cells to Produce IFN-γ upon Exposure to Plasmodium falciparum-Infected.
SIV-specific CD8+ T cells express high levels of PD1 and cytokines but have impaired proliferative capacity in acute and chronic SIVmac251 infection by.

SIV-specific CD8+ T cells express high levels of PD1 and cytokines but have impaired proliferative capacity in acute and chronic SIVmac251 infection by.
Engineering Human Peripheral Blood Stem Cell Grafts that Are Depleted of Naïve T Cells and Retain Functional Pathogen-Specific Memory T Cells  Marie Bleakley,

Engineering Human Peripheral Blood Stem Cell Grafts that Are Depleted of Naïve T Cells and Retain Functional Pathogen-Specific Memory T Cells  Marie Bleakley,
Supplementary Figure 2 Supplementary Figure 2. White blood cell differential count White blood cell (WBC) differential counts were analyzed from patients.

Supplementary Figure 2 Supplementary Figure 2. White blood cell differential count White blood cell (WBC) differential counts were analyzed from patients.
Supplemental Figure 1 Supplemental Figure 1: IL-10 production in positively or negatively selected B cells. B cells were isolated with un-touched (Untouched.

Supplemental Figure 1 Supplemental Figure 1: IL-10 production in positively or negatively selected B cells. B cells were isolated with un-touched (Untouched.
A B C D CD4+ CD8+ Control Isotype Ab Anti-TNFa Ab Control Isotype Ab

A B C D CD4+ CD8+ Control Isotype Ab Anti-TNFa Ab Control Isotype Ab
Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 

Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 
Immune profiling of ZIKV-infected PBMCs

Immune profiling of ZIKV-infected PBMCs

Similar presentations

Ads by Google