Summer Assignment: Virtual Lab Seed Experiment 1. Research Notebook Set-Up (a tutorial for you!) 2. Data Collection 3. Completing Virtual Seed Lab in the Notebook

Virtual Seed Lab Objective: Students will: Set-Up and fill in their Research Notebook. Understand requirements for seeds to germinate and grow; Be able to explain and analyze the growth patterns of seedlings. Recognize differences between plants in their germination and growth requirements

Research Lab Notebook - lets get started: Here is an Overview of how we will set up our Notebooks for every lab: Page 1: Title Page Page 2 -4: Table of Contents Page 5 leave blank (just in case) Page 6 - 13: Label with: Page number Date Title of Lab/experiment OR Research Question? Did you remember to add the date, title and page number to your Table of Contents? If not, do so!

Time to Set Up your Biology Research Notebook! ***We never write on the left page of our Notebook!!!! Title Page: open to your first page (should be on the right side) See next slide: the information needs to be written in the very center of the page! In the top right hand corner write the date. In the bottom right hand corner write the “1” (its your page number!).

Your Name (Jane/John Doe Set up your Title Page: (left side) (leave blank) . (right side) Date Example: Research Biology 2018-2019 Your Name (Jane/John Doe Riverside High School, Leesburg, VA 20176 1.

Time to Set Up your Biology Research Notebook! ***We never write on the left page of our Notebook!!!! Table of Contents: Turn to your next page (right side only!) At the top, in the center, write “Table of Contents” either in ALL CAPS or underlined. In the top right hand corner write the date. In the bottom right hand corner write the “2” (its your page number!).

Does your table of contents look like this? (left side) (Leave blank) . (right side) Date Table of Contents Date Title Page # ___/___/18 Virtual Seed Lab 6-13 2. Set up 3 pages (2 - 4) for the title pages (leave page 5 blank!)

Does your table of contents look like this? (left side) (Leave blank) . (right side) Date Table of Contents Date Title Page # 3. Set up 3 pages (2 - 4) for the title pages (leave page 5 blank!)

Does your table of contents look like this? (left side) (Leave blank) . (right side) Date Table of Contents Date Title Page # 4. Set up 3 pages (2 - 4) for the title pages (leave page 5 blank!)

Set up 3 pages (2 - 4) for the title pages (leave page 5 blank!) Leave this page blank! (left side) (Leave blank) . (right side) 5. Set up 3 pages (2 - 4) for the title pages (leave page 5 blank!)

Notebook Set-Up Cont. Virtual Seed Lab: Research Question & Model Turn to your next page (right side only!) At the top, in the center, write “Virtual Seed Lab” either in ALL CAPS or underlined. In the top right hand corner write the date. In the bottom right hand corner write the “6”. Below the Title, drop down 2-3 lines to write the headers for this page (always start to the left). First Header: Research Question: Second Header: Research Model: (leave a few spaces between headers) See next slide and complete this page.

Lets add our Model to the (left side) (right side) Date VIRTUAL LAB SEED EXPERIMENT 6. Research Question : What do seedlings need to germinate? Research Model : Germinating Seeds water CO2 light

Notebook Set-Up Cont. Virtual Seed Lab: Student Understandings Turn to your next page (right side only!) At the top, write the title and date again. In the bottom right hand corner write “7”. Below the Title, drop down a few spaces and write the Header, “Student Understandings” Repeat for Page 8. See next slides to complete these page. We will fill in this section in class This is where we document background information about the organism(s) and processes we are researching (more to come on this in class!)

Add more pages to this experiment: (left side) (right side) Date VIRTUAL LAB SEED EXPERIMENT 7. STUDENT UNDERSTANDINGS:

Add more pages to this experiment: (left side) (right side) Date VIRTUAL LAB SEED EXPERIMENT 8. STUDENT UNDERSTANDINGS:

Notebook Set-Up Cont. Virtual Seed Lab: Experimental Design Turn to your next page (right side only!) At the top, write the title and date again. In the bottom right hand corner write “9”. Below the Title, drop down a few spaces and write the Header, “Experimental Design” See next slide to set this page.

Add more pages to this experiment: (left side) (right side) Date VIRTUAL LAB SEED EXPERIMENT 9. EXPERIMENTAL DESIGN: Research Question: Null (Ho) Hypothesis: Alternative (Ha) hypothesis: we will add later during class Independent Variable: Experimental Group(s): will fill in while completing experiment in Part 2 of summer assignment Control: Seeds Soaked Overnight (10 hrs light and 14 hrs Dark at 23oC) Dependent Variable: Constants: we will add later during class

Filling out the Experimental Design: Research Question: What do seedlings need to germinate? Hypothesis Null: The Independent Variable (IV) will not effect the Dependent Variable (DV). What is your Independent Variable (IV) for the Virtual Seed Lab? This is going to vary and be based on your last name. See next slide. What is the Dependent Variable (DV)? Measurement of root and shoot length (cm)

Filling out the Experimental Design: What is your Independent Variable (IV) for the Virtual Seed Lab? This is going to be based on your last name. Instructions for these will come in next slides. Last Name Independent Variable Control vs Experiment Group A-E Time (exposed to light) A1 10 hrs light, 14 hrs dark vs. B1 24 hrs dark F-K A1 10 hrs light, 14 hrs dark vs. C1 24 hrs light L-O Type of Light A1 10 hrs light, 14 hrs dark vs. D1 Daylight P-T Rotation of seeds A1 soaked, not rotated vs. A2 soaked & rotated U-Z Presence of water A1 soaked vs. A3 not soaked

Notebook Set-Up Cont. Virtual Seed Lab: Materials & Procedures Turn to your next page (right side only!) At the top, write the title and date again. In the bottom right hand corner write “10”. Below the Title, drop down a few spaces and write the Header, “Materials”. Leave 10 lines and write the Header “Procedures”. MATERIALS SHOULD BE BULLETED PROCEDURES SHOULD BE NUMBERED See next slide for this page.

Add more pages to this experiment: (left side) (right side) Date VIRTUAL LAB SEED EXPERIMENT 10. MATERIALS: (bullet list) PROCEDURES: (numbered steps)

List of Materials to write into Notebook: Seed packets (this experiment uses Peas, Little Marvel) Re-sealable plastic bags (sandwich or quart size) Paper towels or napkins Water Tape Cardboard (optional) Paper clips (optional) Binder clips (optional) Scissors

Procedures to Write into Notebook: Day 1, soak the seeds (this experiment uses Peas, Little Marvel) overnight in water. Day 2, Place a wet paper towel in a ziplock bag and place 5-10 seeds on the paper towel in the bag. Blow air into the bag and seal. On Day 3-4, observe the growth of the seeds.

Notebook Set-Up Cont. Virtual Seed Lab: Data Tables Turn to your next page (right side only!) At the top, write the title and date again. In the bottom right hand corner write “11”. Below the Title, drop down a few spaces and write the Header, “Data Table”. See next slide for this page.

Add more pages to this experiment: (left side) (right side) Date VIRTUAL LAB SEED EXPERIMENT 11. DATA TABLES: be sure to include a Title for the tables Titles are always “Table 1 (or 2 if you have multiple tables): The effect of IV on DV”

Notebook Set-Up Cont. Virtual Seed Lab: Graphs Turn to your next page (right side only!) At the top, write the title and date again. In the bottom right hand corner write “12”. Below the Title, drop down a few spaces and write the Header, “Graph”. See next slide for this page.

Add more pages to this experiment: (left side) (right side) Date VIRTUAL LAB SEED EXPERIMENT 12. GRAPHS: 2 Graphs! 1 for ROOTS and 1 for SHOOTS Be sure to include the following for your Graph: Title (The Effect of the IV on the DV) Dependent variable on Y axis Independent variable on X axis Axis labeled Scales drawn on axis Key included Drawn neatly (with a ruler – no free Hand drawing of your graph)

Notebook Set-Up Cont. Virtual Seed Lab: Results Paragraph Turn to your next page (right side only!) At the top, write the title and date again. In the bottom right hand corner write “13”. Below the Title, drop down a few spaces and write the Header, “Results”. See next slides for guidance on this page.

Analysis of Data/Results Making a Claim from the Data for the Control seeds and the experimental seeds on days 1-10: Look at your graphs. Do you notice any trends in the data? Which days do the seeds start to grow a root, or a shoot? Do roots and shoots grow at the same rate? Does the data help us to answer our research questions: How do seedlings develop over time? See next slide for further guidance on how to compose your results paragraph.

Add more pages to this experiment: (left side) (right side) Date VIRTUAL LAB SEED EXPERIMENT 13. Results: (in paragraph form) State your Research Question. Claim: Make a claim - (statement that answers the research question) Evidence: Provide evidence from the data that was collected Reasoning - describe how the science principles explain the results

*** be sure to go back and fill in the information on your Table of Contents!!!*** Move on to Part 3: Collect your Data All Done with Part 1!!! This direction sheet can be found in the Summer Assignment Packet